Wills J W, Troutman W B, Riggsby W S
J Bacteriol. 1985 Oct;164(1):7-13. doi: 10.1128/jb.164.1.7-13.1985.
The mitochondrial DNA (mtDNA) of the dimorphic fungus Candida albicans has a molecular size of 41 kilobase pairs as judged by summation of the fragment sizes produced by digestion with restriction endonucleases EcoRI, PvuII, and a combination of both enzymes. Five of the six EcoRI fragments comprising the mitochondrial genome have been cloned into the plasmid vector, pBR322. Restriction mapping revealed a circular map as predicted by previous observations with the electron microscope. The use of nick-translated, purified mtDNA to probe digests of mtDNA from other strains of C. albicans revealed a common restriction pattern. Use of nick-translated, cloned EcoRI fragments to probe digests of mtDNA revealed a large (at least 5 kilobase pairs), inverted duplication as well as a smaller (less than 0.4 kilobase pairs) region of related sequences.
通过对经限制性内切酶EcoRI、PvuII以及这两种酶联合消化产生的片段大小进行求和判断，二态真菌白色念珠菌的线粒体DNA（mtDNA）分子大小为41千碱基对。构成线粒体基因组的六个EcoRI片段中的五个已被克隆到质粒载体pBR322中。限制性酶切图谱显示为环形图谱，这与之前用电子显微镜观察的结果一致。使用缺口平移法纯化的mtDNA来探测其他白色念珠菌菌株的mtDNA消化产物，揭示了一种常见的限制性模式。使用缺口平移法克隆的EcoRI片段来探测mtDNA消化产物，揭示了一个大的（至少5千碱基对）反向重复序列以及一个较小的（小于0.4千碱基对）相关序列区域。
