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通过功能筛选出的由大肠杆菌RNA聚合酶识别的启动子：来自噬菌体T5的高效启动子。

Promoters recognized by Escherichia coli RNA polymerase selected by function: highly efficient promoters from bacteriophage T5.

作者信息

Gentz R, Bujard H

出版信息

J Bacteriol. 1985 Oct;164(1):70-7. doi: 10.1128/jb.164.1.70-77.1985.

DOI:10.1128/jb.164.1.70-77.1985
PMID:3900050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214212/
Abstract

Highly efficient promoters of coliphage T5 were identified by selecting for functional properties. Eleven such promoters belonging to all three expression classes of the phage were analyzed. Their average AT content was 75% and reached 83% in subregions of the sequences. Besides the well-known conserved sequences around -10 and -33, they exhibited homologies outside the region commonly considered to be essential for promoter function. Interestingly, the consensus hexamers around -10 (TAT AAT) and -35 (TTG ACA) were never found simultaneously within the sequence of highly efficient promoters. Several of these promoters compete extremely well for Escherichia coli RNA polymerase and can be used for the efficient in vitro synthesis of defined RNA species. In addition, some of these promoters accept 7-mGpppA as the starting dinucleotide, thus producing capped mRNA in vitro which can be utilized in various eucaryotic translation systems.

摘要

通过筛选功能特性鉴定出了大肠杆菌噬菌体T5的高效启动子。分析了属于该噬菌体所有三种表达类别的11个此类启动子。它们的平均AT含量为75%，在序列的子区域中达到83%。除了-10和-33附近众所周知的保守序列外，它们在通常认为对启动子功能至关重要的区域之外还表现出同源性。有趣的是，在高效启动子序列中从未同时发现-10（TAT AAT）和-35（TTG ACA）周围的共有六聚体。其中几个启动子与大肠杆菌RNA聚合酶竞争非常激烈，可用于体外高效合成特定的RNA种类。此外，其中一些启动子接受7-mGpppA作为起始二核苷酸，从而在体外产生可在各种真核翻译系统中使用的加帽mRNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/b00a93232a57/jbacter00215-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/984bea196cda/jbacter00215-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/d0f4b6e22e57/jbacter00215-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/2feae65c09a8/jbacter00215-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/b0980530767a/jbacter00215-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/ec78e6414544/jbacter00215-0085-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/b00a93232a57/jbacter00215-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/984bea196cda/jbacter00215-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/d0f4b6e22e57/jbacter00215-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/2feae65c09a8/jbacter00215-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/b0980530767a/jbacter00215-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/ec78e6414544/jbacter00215-0085-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/40f6/214212/b00a93232a57/jbacter00215-0086-a.jpg

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