State Key Laboratory of Bio-Control, School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Key Laboratory of Pharmaceutical Functional Genes, Sun Yat-sen University, Guangzhou 510275, China.
Int J Mol Sci. 2024 Jul 7;25(13):7450. doi: 10.3390/ijms25137450.
The effects of intestinal microflora on extraintestinal immune response by intestinal cytokines and metabolites have been documented, but whether intestinal microbes stimulate serum antibody generation is unknown. Here, serum antibodies against 69 outer membrane proteins of , a dominant bacterium in the human intestine, are detected in 141 healthy individuals of varying ages. Antibodies against outer membrane proteins are determined in all serum samples tested, and frequencies of antibodies to five outer membrane proteins (OmpA, OmpX, TsX, HlpA, and FepA) are close to 100%. Serum antibodies against outer membrane proteins are further validated by Western blot and bacterial pull-down. Moreover, the present study shows that OstA, HlpA, Tsx, NlpB, OmpC, YfcU, and OmpA provide specific immune protection against pathogenic , while HlpA and OmpA also exhibit cross-protection against infection. These finding indicate that intestinal activate extraintestinal antibody responses and provide anti-infective immunity.
肠道微生物通过细胞因子和代谢物对肠外免疫反应的影响已被证实，但肠道微生物是否能刺激血清抗体产生尚不清楚。本研究检测了 141 名不同年龄的健康个体血清中针对肠道优势菌 69 种外膜蛋白的抗体。在所有检测的血清样本中均检测到针对 外膜蛋白的抗体，且对 5 种外膜蛋白（OmpA、OmpX、TsX、HlpA 和 FepA）的抗体频率接近 100%。Western blot 和细菌下拉实验进一步验证了针对 外膜蛋白的血清抗体。此外，本研究表明 OstA、HlpA、Tsx、NlpB、OmpC、YfcU 和 OmpA 可针对致病性 提供特异性免疫保护，而 HlpA 和 OmpA 也对 感染具有交叉保护作用。这些发现表明肠道 可激活肠外抗体反应并提供抗感染免疫力。
