Sun Zeyang, Li Haihua, Li Yupeng, Qiao Jiayun
Tianjin Key Laboratory of Conservation and Utilization of Animal Diversity, College of Life Sciences, Tianjin Normal University, Tianjin, China.
Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
Front Vet Sci. 2020 Dec 16;7:547425. doi: 10.3389/fvets.2020.547425. eCollection 2020.
Intestinal health is a critical issue for piglets during their weaning transition period. Previous reports have emphasized the promise of distinct probiotics in improving the enteric health. Here in this research, a newly isolated strain was pretreated to Lipopolysaccharide (LPS)-challenged piglets and its association with integrity of the intestinal barrier coupled with effective dosage were expected to be signified. In the present study, 72 piglets (Landrace × Yorkshiere × Duroc) were randomly allotted to four groups, each group with six replicates. The subjects in the control group were provided with basal diet while those in other tested groups with extra 0.05, 0.1, and 0.2% , respectively. Fourteen days later, LPS was intraperitoneally injected and sodium pentobarbital was then delivered to euthanize those LPS-challenged piglets. An increase of average daily gain and body weight along with an apparent decline of diarrhea rate were observed in -treated groups. Both 0.1 and 0.2% supplement in total diet had the capability to markedly elevate levels of CAT, GSH-Px, SOD, anti-inflammatory cytokine from the serum as well as tight junction proteins (Claudin-1, Occludin, and ZO-1) extracted from intestine in LPS-challenged piglets. These changes were accompanied by the obvious downregulation of D-lactic acid, DAO, MDA and pro-inflammatory mediators in the serum, including IL-1β, IL-6, IFN-γ, and TNF-α. Meanwhile, the expression levels of TLR2 and TLR4 in spleen and mesenteric lymph nodes were significantly lower whereas the oxidation-related gene, was up-regulated with administration. Our findings suggested that relatively high dose (0.1-0.2%) could regulate the progression of inflammatory response and oxidative stress when individuals were exposed to LPS, thus probably offering valuable assistance in restoring barrier function and improving overall performance.
肠道健康是仔猪断奶过渡期的一个关键问题。先前的报道强调了不同益生菌在改善肠道健康方面的前景。在本研究中，一种新分离的菌株被用于预处理脂多糖（LPS）攻击的仔猪，预期其与肠道屏障完整性的关联以及有效剂量将得到明确。在本研究中，72头仔猪（长白×大白×杜洛克）被随机分为四组，每组六个重复。对照组的仔猪喂食基础日粮，而其他试验组分别额外添加0.05%、0.1%和0.2%。十四天后，腹腔注射LPS，然后注射戊巴比妥钠对受LPS攻击的仔猪实施安乐死。在处理组中观察到平均日增重和体重增加，腹泻率明显下降。日粮中添加0.1%和0.2%均能够显著提高受LPS攻击仔猪血清中CAT、GSH-Px、SOD、抗炎细胞因子以及从肠道中提取的紧密连接蛋白（Claudin-1、Occludin和ZO-1）的水平。这些变化伴随着血清中D-乳酸、DAO、MDA以及促炎介质（包括IL-1β、IL-6、IFN-γ和TNF-α）的明显下调。同时，脾脏和肠系膜淋巴结中TLR2和TLR4的表达水平显著降低，而氧化相关基因在添加后上调。我们的研究结果表明，相对高剂量（0.1-0.2%）在个体暴露于LPS时能够调节炎症反应和氧化应激的进程，从而可能为恢复屏障功能和改善整体性能提供有价值的帮助。
