在猪模型中调节肠道屏障功能和肠道微生物群。
Modulates Intestinal Barrier Function and Gut Microbiota in a Pig Model.
作者信息
Zheng Hongmei, Cao Haigang, Zhang Deming, Huang Jiahe, Li Jinshu, Wang Shaoying, Lu Junfeng, Li Xiao, Yang Gongshe, Shi Xin'e
机构信息
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
出版信息
Front Microbiol. 2022 Mar 17;13:810230. doi: 10.3389/fmicb.2022.810230. eCollection 2022.
This study investigated the effects of (CM) on intestinal barrier function and gut microbiota in a pig model. A total of 160 pigs were randomly allocated to either a control group (fed the basal diet) or a CM group (fed the basal diet supplemented with 300 mg/kg CM). CM improved intestinal morphology and increased the numbers of goblet cells and intraepithelial lymphocytes. CM also elevated the expression of zona occluden-1, claudin-1, mucin-2 and secretory immunoglobulin A. Furthermore, the mucosal levels of pro-inflammatory cytokines were downregulated while the levels of anti-inflammatory cytokines were upregulated in the CM group. Mechanistically, CM downregulated the expression of key proteins of the TLR4/MyD88/NF-κB signaling pathway. Moreover, CM altered the colonic microbial composition and increased the concentrations of acetate and butyrate. In conclusion, CM can modulate the intestinal barrier function and gut microbiota, which may provide a new strategy for improving intestinal health.
本研究在猪模型中探究了（某种物质，原文未明确写出全称，推测可能是某种化合物之类的，用“（CM）”表示）对肠道屏障功能和肠道微生物群的影响。总共160头猪被随机分为对照组（饲喂基础日粮）或CM组（饲喂添加300毫克/千克CM的基础日粮）。CM改善了肠道形态，增加了杯状细胞和上皮内淋巴细胞的数量。CM还提高了闭合蛋白-1、紧密连接蛋白-1、黏蛋白-2和分泌型免疫球蛋白A的表达。此外，CM组促炎细胞因子的黏膜水平下调，而抗炎细胞因子的水平上调。从机制上讲，CM下调了TLR4/MyD88/NF-κB信号通路关键蛋白的表达。此外，CM改变了结肠微生物组成，增加了乙酸盐和丁酸盐的浓度。总之，CM可以调节肠道屏障功能和肠道微生物群，这可能为改善肠道健康提供一种新策略。
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