Mandodan Sahadiya, Gangmei Kakhuangailiu, Vijayakumar Abhisubesh, Kunnikuruvan Aneha, Lukose Jibi, Padmanaban Hemaladkshmi, Bora Bhagyashree, Ashokkumar Mathivanan, Irudayaraj Geetha, Subbiah Poopathi
Unit of Microbiology and Immunology (UMI), Department of Health Research (Ministry of Health & Family Welfare, Govt. of India), ICMR-Vector Control Research Centre (VCRC), Medical Complex Indira Nagar, Puducherry, 605006, India.
Mol Biol Rep. 2024 Jul 13;51(1):800. doi: 10.1007/s11033-024-09734-9.
Mosquitoes are widespread globally and have contributed to transmitting pathogens to humans and the burden of vector-borne diseases. They are effectively controlled at their larval stages by biocontrol agents. Unravelling natural sources for microbial agents can lead us to novel potential candidates for managing mosquito-borne diseases. In the present study, an attempt was made to isolate a novel bacterium from the field-collected agricultural soil for larvicidal activity and promising bacterial metabolites for human healthcare.
Field-collected soil samples from the Union territory of Puducherry, India, have been used as the source of bacteria. Isolate VCRC B655 belonging to the genus Lysinibacillus was identified by 16S rRNA gene sequencing and exhibited promising larvicidal activity against different mosquito species, including Culex (Cx.) quinquefasciatus, Anopheles (An.) stephensi, and Aedes (Ae.) aegypti. The lethal concentration (LC) of Lysinibacillus sp. VCRCB655 was observed to be high for Cx. quiquefasciatus: LC at 0.047 mg/l, LC at 0.086 mg/l, followed by An. stephensi and Ae. aegypti (LC: 0.6952 mg/l and 0.795 mg/l) respectively. Additionally, metabolic profiling of the culture supernatant was carried out through Gas chromatography and Mass spectrophotometry (GC/MS) and identified 15 major secondary metabolites of different metabolic classes. Diketopiperazine (DKPs), notably pyro lo [1, 2-a] pyrazine1, 4-dione, are the abundant compounds reported for antioxidant activity, and an insecticide compound benzeneacetic acid was also identified.
A new bacterial isolate, Lysinibacillus sp. VCRC B655 has been identified with significant larvicidal activity against mosquito larvae with no observed in non-target organisms. GC-MS analysis revealed diverse bioactive compounds with substantial biological applications. In conclusion, Lysinibacillus sp. VCRC B655 showed promise as an alternative biocontrol agent for mosquito vector control, with additional biological applications further enhancing its significance.
蚊子在全球广泛分布，导致病原体传播给人类，增加了媒介传播疾病的负担。它们在幼虫阶段可通过生物防治剂得到有效控制。揭示微生物制剂的天然来源可引导我们找到控制蚊媒疾病的新型潜在候选物。在本研究中，我们尝试从田间采集的农业土壤中分离一种新型细菌，用于杀幼虫活性研究，并寻找对人类健康有益的有前景的细菌代谢产物。
从印度本地治里联邦属地采集的田间土壤样本被用作细菌来源。通过16S rRNA基因测序鉴定出属于赖氨酸芽孢杆菌属的分离株VCRC B655，它对不同蚊种，包括致倦库蚊（Culex (Cx.) quinquefasciatus）、斯氏按蚊（Anopheles (An.) stephensi）和埃及伊蚊（Aedes (Ae.) aegypti）表现出有前景的杀幼虫活性。赖氨酸芽孢杆菌属VCRCB655对致倦库蚊的致死浓度（LC）较高：LC为0.047毫克/升，对斯氏按蚊和埃及伊蚊的LC分别为0.086毫克/升、0.6952毫克/升和0.795毫克/升。此外，通过气相色谱和质谱联用（GC/MS）对培养上清液进行代谢谱分析，鉴定出15种不同代谢类别的主要次生代谢产物。二酮哌嗪（DKPs），特别是吡咯并[1, 2 - a]吡嗪 - 1, 4 - 二酮，是报道具有抗氧化活性的丰富化合物，还鉴定出一种杀虫剂化合物苯乙酸。
已鉴定出一种新的细菌分离株，赖氨酸芽孢杆菌属VCRC B655，对蚊幼虫具有显著的杀幼虫活性，且在非靶标生物中未观察到该活性。GC - MS分析揭示了具有大量生物学应用的多种生物活性化合物。总之，赖氨酸芽孢杆菌属VCRC B655有望作为控制蚊媒的替代生物防治剂，其额外的生物学应用进一步增强了其重要性。
