Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China; Collaborative Innovation Center of Energy Conservation & Emission Reduction and Sustainable Urban-Rural Development in Beijing, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
Centre for Urban Environmental Remediation, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China; Collaborative Innovation Center of Energy Conservation & Emission Reduction and Sustainable Urban-Rural Development in Beijing, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
J Environ Manage. 2024 Aug;366:121803. doi: 10.1016/j.jenvman.2024.121803. Epub 2024 Jul 14.
In this work, a novel polyurethane carrier modified with biochar and tourmaline/zeolite powder at ratio of 1:1 and 1:2 was developed to promote the formation of biofilms and the synergy of overall bacterial activity for Partial Denitrification/Anammox to treat low-nitrogen contaminated surface water. Based on the batch experiment, the modified biocarrier, BTP2 (biochar: tourmaline = 2: 1), exhibited the highest total nitrogen removal efficiency (83.63%) under influent total nitrogen of 15 mg/L and COD/NO of 3. The dense biofilm was formed in inner side of biocarrier owing to the increased surface roughness and various functional groups suggested by scanning electron microscopy and Fourier-transform infrared analysis. The EPS content increased from 200.15 to 220.26 mg/g VSS in BTP2 system. Besides, the rapid NH capture and organics release of the modified carrier fueled the growth of anammox and denitrification bacteria, with the activity of 2.13 ± 0.52 mg N/gVSS/h and 6.70 ± 0.52 mg N/gVSS/h (BTP2). High-throughput sequencing unraveled the increased abundances of Candidatus_Competibacter (0.82%), Thauera (0.60%) and Candidatus_Brocadia (0.55%) which was responsible for the synergy of incomplete reduction of NO to NO and NH oxidation. Overall, this study provided a valid and simple-control guide for biofilm formation towards rapid enrichment and great collaboration of Anammox and denitrification bacteria.
在这项工作中，开发了一种新型的聚氨酯载体，用生物炭和电气石/沸石粉以 1:1 和 1:2 的比例进行修饰，以促进生物膜的形成和整体细菌活性的协同作用，从而实现低氮污染地表水的部分反硝化/厌氧氨氧化处理。基于批处理实验，在进水总氮为 15mg/L 和 COD/NO 为 3 的条件下，改性生物炭 BTP2（生物炭：电气石=2:1）表现出最高的总氮去除效率（83.63%）。扫描电子显微镜和傅里叶变换红外分析表明，由于表面粗糙度增加和各种官能团的存在，生物载体内部形成了致密的生物膜。BTP2 系统中的 EPS 含量从 200.15 增加到 220.26mg/gVSS。此外，改性载体对 NH 的快速捕获和有机物的释放促进了厌氧氨氧化和反硝化细菌的生长，其活性分别为 2.13±0.52mgN/gVSS/h 和 6.70±0.52mgN/gVSS/h（BTP2）。高通量测序揭示了 Candidatus_Competibacter（0.82%）、Thauera（0.60%）和 Candidatus_Brocadia（0.55%）的丰度增加，它们负责不完全还原 NO 到 NO 和 NH 氧化的协同作用。总的来说，这项研究为生物膜的形成提供了一个有效的、简单控制的指导，有助于快速富集和促进厌氧氨氧化和反硝化细菌的协同作用。
