Parr E L, Kirby W N
J Histochem Cytochem. 1979 Oct;27(10):1327-36. doi: 10.1177/27.10.390033.
This report describes an immunoferritin labeling study of mouse H-2 histocompatibility antigens on epithelial cells dissociated from stomach, duodenum-jejunum, ileum, trachea, diestrus uterus, gall bladder, and vas deferens. Before cell dissociation, most of the organs were prefixed in periodate-lysine-paraformaldehyde to preserve the shape of the cells and to immobilize H-2 antigens in their native positions. Five kinds of epithelial cells expressed H-2 antigens on lateral and basal membranes but not on apical membranes. These were the lining cells of the upper intestine, ileum, gall gladder, uterus, and the tracheal brush cell. The antigens were continuously distributed on the lateral and basal membranes of these cells and appeared to be absent from the apical membranes, rather than masked by the fuzzy coat. On four other epithelial cell types H-2 antigens could not be detected. These were the lining cells of the vas deferens, parietal and chief cells from the stomach, and ciliated tracheal cells. It does not seem to be uncommon for normal nucleated cells to lack H-2 antigens. On fixed and labeled epithelial cells from the upper intestine the zonula occludens membranes were unlabeled, while the zonula adherens and desmosome membranes were labeled as densely as the remainder of the lateral membranes. The zonula occludens membrane thus constituted the boundary betewen the unlabeled apical membrane and the labeled lateral membrane of these cells. Intestinal epithelial cells dissociated without prefixation showed a patchy distribution of H-2 antigens on their lateral membranes after indirect labeling, indicating antigen mobility in this membrane. On the same unfixed dissociated cells the antigens were able to migrate from lateral to apical membranes, a movement which appears to be prevented in the intact epithelial layer by the occluding junction. The absence of H-2 antigens from apical membranes and their inability to migrate through an intact zonula occludens suggest that these molecules must reach the lateral membranes of epithelial cells by a pathway which is distinct from that followed by apical membrane components.
本报告描述了一项免疫铁蛋白标记研究，该研究针对从小鼠胃、十二指肠 - 空肠、回肠、气管、动情期子宫、胆囊和输精管中分离出的上皮细胞上的H - 2组织相容性抗原。在细胞解离之前，大多数器官用高碘酸盐 - 赖氨酸 - 多聚甲醛进行预固定，以保持细胞形状并将H - 2抗原固定在其原位。五种上皮细胞在侧面和基底膜上表达H - 2抗原，但在顶端膜上不表达。这些细胞是上肠道、回肠、胆囊、子宫的内衬细胞以及气管刷细胞。抗原在这些细胞的侧面和基底膜上连续分布，并且似乎在顶端膜上不存在，而不是被模糊的被膜掩盖。在其他四种上皮细胞类型上未检测到H - 2抗原。这些细胞是输精管的内衬细胞、胃的壁细胞和主细胞以及气管纤毛细胞。正常有核细胞缺乏H - 2抗原似乎并不罕见。在上肠道的固定和标记上皮细胞上，紧密连接膜未被标记，而黏着连接和桥粒膜与侧面膜的其余部分一样被密集标记。因此，紧密连接膜构成了这些细胞未标记的顶端膜和标记的侧面膜之间的边界。未进行预固定而解离的肠道上皮细胞在间接标记后，其侧面膜上显示出H - 2抗原的斑块状分布，表明该膜中抗原具有流动性。在相同的未固定解离细胞上，抗原能够从侧面膜迁移到顶端膜，而在完整的上皮层中，这种迁移似乎被紧密连接所阻止。顶端膜上缺乏H - 2抗原以及它们无法穿过完整的紧密连接迁移，这表明这些分子必须通过一条与顶端膜成分所遵循的途径不同的途径到达上皮细胞的侧面膜。
