Kirby W N, Parr E L
J Histochem Cytochem. 1979 Mar;27(3):746-50. doi: 10.1177/27.3.479552.
This report describes an immunoferritin labeling study of mouse H-2 histocompatibility antigens on epithelial cells dissociated from the small intestine by EDTA and trypsin. Before cell dissociation, the intestine was prefixed in paraformaldehyde or periodate-lysine-paraformaldehyde in order to preserve the shape of the cells and to immobilize H-2 antigens in their native positions. The results demonstrated the presence of H-2 antigens on the lateral and basal cell membranes at about the same high density that was observed at the surface of mouse monocytes. No H-2 antigens could be detected at the apical surface of dissociated or undissociated epithelial cells. It is unlikely that the fuzzy coat masked H-2 antigens at the apical surface because it was essentially absent from the apical membranes of dissociated cells. These observations extend our knowledge of the cellular distribution of transplantation antigens, and provide further evidence of a discontinuity in the expression of membrane components at the junctional complex of epithelial cells.
本报告描述了一项免疫铁蛋白标记研究，该研究针对通过乙二胺四乙酸（EDTA）和胰蛋白酶从小肠解离的上皮细胞上的小鼠H-2组织相容性抗原展开。在细胞解离之前，将小肠用多聚甲醛或高碘酸盐-赖氨酸-多聚甲醛进行预固定，以保持细胞形状并将H-2抗原固定在其原位。结果表明，在侧向和基底细胞膜上存在H-2抗原，其密度与在小鼠单核细胞表面观察到的大致相同。在解离或未解离的上皮细胞的顶端表面均未检测到H-2抗原。顶端表面的模糊被膜不太可能掩盖H-2抗原，因为在解离细胞的顶端膜上基本不存在该被膜。这些观察结果扩展了我们对移植抗原细胞分布的认识，并进一步证明了上皮细胞连接复合体处膜成分表达的不连续性。
