Université Paris-Saclay, INRAE, PROSE, 92761, Antony, France.
Université Paris-Saclay, INRAE, MaIAGE, 78350, Jouy-en-Josas, France.
Curr Microbiol. 2024 Jul 14;81(9):266. doi: 10.1007/s00284-024-03774-x.
DNA Stable Isotope Probing is emerging as a potent methodology for investigating host-virus interactions, based on the essential reliance of viruses on host organisms for the production of virions. Despite the anticipated link between host isotopic compositions and the generated virions, the application of stable isotope probing to viral DNA has never been evaluated on simple biological models. In this study, we assessed the efficacy of this method on the bacteriophage T4 and its host, Escherichia coli. Through the cultivation of E. coli cells on a C-enriched substrate and subsequent propagation of T4 bacteriophage, we examine the degree of isotopic enrichment in viral DNA. Our investigation reveals a strong correlation between the proportion of C-D-glucose in the growth substrate and the buoyant density in CsCl gradient of T4 DNA, confirming the validity of DNA SIP in viral ecology. These findings underscore the potential of DNA SIP as a robust tool for characterizing the diversity of viruses infecting hosts with specific metabolic activities and provide then a foundation for further exploration in viral ecology research.
DNA 稳定同位素探测技术正逐渐成为研究宿主-病毒相互作用的有力方法，这是基于病毒在生产病毒粒子时对宿主生物的绝对依赖。尽管宿主同位素组成与生成的病毒粒子之间存在预期的联系，但稳定同位素探测技术从未在简单的生物模型上应用于病毒 DNA。在这项研究中，我们评估了该方法在噬菌体 T4 及其宿主大肠杆菌中的效果。通过在富含 C 的底物上培养大肠杆菌细胞，然后增殖 T4 噬菌体，我们研究了病毒 DNA 中同位素富集的程度。我们的研究表明，生长基质中 C-D-葡萄糖的比例与 T4 DNA 在 CsCl 梯度中的浮力密度之间存在很强的相关性，这证实了 DNA SIP 在病毒生态学中的有效性。这些发现强调了 DNA SIP 作为一种强大工具的潜力，可用于描述具有特定代谢活性的宿主感染病毒的多样性，并为病毒生态学研究提供了进一步探索的基础。
