Zhang H J, Zhang E M, He J R, Li W, Wei J C
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2024 Jul 10;45(7):990-996. doi: 10.3760/cma.j.cn112338-20231212-00350.
To analyze the multiple locus variable number tandem repeat analysis (MLVA) genotype polymorphism of and establish a MLVA genotype database of in China. strains isolated from different sources in China since 1947 were collected. Genotype identification was carried out using the MLVA15 genotyping protocol based on 15 variable number tandem repeat loci. The genotypes were uniformly numbered and named. The distribution characteristics of the MLVA genotypes of strains were analyzed. Software Bionumerics was used to construct clustering diagrams to analyze the genetic relationships. The MLVA15 clustering analysis subdivided the isolates into 4 major groups and 91 genotypes, 54 of which were unique to China. The genotypes from MLVA15-CHN1 to MLVA15-CHN6 were widely distributed throughout China and in all eras, while other genotypes were restricted to certain regions or eras. This study established a MLVA genotype database of , which provides basis for the understanding of MLVA genetic polymorphisms and the control and molecular source tracing of the anthrax outbreaks in China.
分析炭疽芽孢杆菌多位点可变数目串联重复序列分析（MLVA）基因型多态性，建立中国炭疽芽孢杆菌MLVA基因型数据库。收集1947年以来中国不同来源分离的炭疽芽孢杆菌菌株。采用基于15个可变数目串联重复序列位点的MLVA15基因分型方案进行基因型鉴定。对基因型进行统一编号和命名。分析炭疽芽孢杆菌菌株MLVA基因型的分布特征。使用Bionumerics软件构建聚类图以分析遗传关系。MLVA15聚类分析将分离株分为4个主要组和91种基因型，其中54种为中国特有。MLVA15-CHN1至MLVA15-CHN6基因型在中国各地及各个时期均广泛分布，而其他基因型则局限于特定地区或时期。本研究建立了炭疽芽孢杆菌MLVA基因型数据库，为了解MLVA遗传多态性以及中国炭疽疫情的防控和分子溯源提供了依据。
