Larijanian Laleh, Shafiei Morvarid, Ghasemi Pirbalouti Abdollah, Ferdousi Atousa, Chiani Mohsen
Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Iran J Microbiol. 2024 Jun;16(3):366-375. doi: 10.18502/ijm.v16i3.15794.
Methicillin-resistant (MRSA) is a major cause of nosocomial and community acquired infections. Nanoparticles are considered as proper tools to overcome the therapeutic problem of antimicrobial-resistant infections because of the drug concentration increment at the desired location and protection from enzymatic degradation. The goal of this study was to evaluate the effect of the antibacterial and antibiofilm activities of zingerone and niosome containing zingerone against pre-formed biofilm of MRSA isolates.
62 MRSA isolates cultured from patients with diabetic ulcers were investigated. Niosomes were synthesized and characterized by X-ray diffraction, zeta potential and scanning electron microscopy (SEM). The size of niosomal particles measured by SEM and zetasizer.
The surface charge of prepared niosomes was about -37 mV. The effect of the zingerone and noisome containing zingerone was evaluated against biofilms of MRSA isolates. Also, the antibiofilm activity of prepared niosomes on gene expression of MRSA biofilms was evaluated using Real Time PCR. Our results demonstrated that the niosome containing zingerone had a diameter of 196.1 nm and a -37.3-mV zeta potential. Zingerone removed one and three-day old biofilms of MRSA at the concentration of 1000 μg/ml, while the zingerone-laoded niosomes removed 1, 3- and 5-days old biofilms at the concentration of 250 μg/ml, 250 μg/ml, and 500 μg/ml.
The results indicated that niosome containing zingerone eliminated MRSA and its biofilms faster compared with free zingerone and it suggested that zingerone-encapsulated niosomes could be considered as a promising treatment against MRSA and its biofilms.
耐甲氧西林金黄色葡萄球菌（MRSA）是医院获得性感染和社区获得性感染的主要原因。纳米颗粒因其能在所需位置提高药物浓度并防止酶降解，被视为克服抗菌耐药性感染治疗难题的合适工具。本研究的目的是评估姜辣素及含姜辣素的脂质体对MRSA分离株形成的成熟生物膜的抗菌和抗生物膜活性。
对从糖尿病溃疡患者中培养出的62株MRSA分离株进行研究。通过X射线衍射、zeta电位和扫描电子显微镜（SEM）对脂质体进行合成与表征。用SEM和粒度分析仪测量脂质体颗粒的大小。
制备的脂质体表面电荷约为-37 mV。评估了姜辣素及含姜辣素的脂质体对MRSA分离株生物膜的作用。此外，使用实时定量PCR评估制备的脂质体对MRSA生物膜基因表达的抗生物膜活性。我们的结果表明，含姜辣素的脂质体直径为196.1 nm，zeta电位为-37.3 mV。姜辣素在浓度为1000 μg/ml时可去除MRSA的1日龄和3日龄生物膜，而载有姜辣素的脂质体在浓度为250 μg/ml、250 μg/ml和500 μg/ml时可去除1日龄、3日龄和5日龄生物膜。
结果表明，与游离姜辣素相比，含姜辣素的脂质体能更快地消除MRSA及其生物膜，这表明包封姜辣素的脂质体可被视为一种有前景的抗MRSA及其生物膜的治疗方法。
