Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA, 01003, USA.
Department of Biomedical Engineering, University of Massachusetts Amherst, 240 Thatcher Road, Amherst, MA, 01003, USA.
Adv Healthc Mater. 2022 Nov;11(21):e2201060. doi: 10.1002/adhm.202201060. Epub 2022 Sep 9.
Bacterial biofilms are a major healthcare concern resulting in refractory conditions such as chronic wounds, implant infections and failure, and multidrug-resistant infections. Aggressive and invasive strategies are employed to cure biofilm infections but are prone to long and expensive treatments, adverse side-effects, and low patient compliance. Recent strategies such as ultrasound-based therapies and antimicrobial nanomaterials have shown some promise in the effective eradication of biofilms. However, maximizing therapeutic effect while minimizing healthy tissue damage is a key challenge that needs to be addressed. Here a combination treatment involving ultrasound and antimicrobial polymeric nanoparticles (PNPs) that synergistically eradicate bacterial biofilms is reported. Ultrasound treatment rapidly disrupts biofilms and increases penetration of antimicrobial PNPs thereby enhancing their antimicrobial activity. This results in superior biofilm toxicity, while allowing for a two- to sixfold reduction in both the concentration of PNPs as well as the duration of ultrasound. Furthermore, that this reduction minimizes cytotoxicity toward fibroblast cells, while resulting in a 100- to 1000-fold reduction in bacterial concentration, is demonstrated.
细菌生物膜是一个主要的医疗保健问题，导致难治性疾病，如慢性伤口、植入物感染和失败，以及多药耐药感染。为了治疗生物膜感染，采用了激进和侵袭性的策略，但容易导致长期和昂贵的治疗、不良反应和低患者依从性。最近的策略，如基于超声的治疗和抗菌纳米材料，在有效清除生物膜方面显示出了一些希望。然而，最大限度地提高治疗效果，同时最小化健康组织损伤是一个需要解决的关键挑战。在这里，报告了一种涉及超声和抗菌聚合物纳米颗粒（PNP）的联合治疗方法，该方法协同根除细菌生物膜。超声处理能迅速破坏生物膜并增加抗菌 PNP 的穿透性，从而增强其抗菌活性。这导致了更好的生物膜毒性，同时允许 PNP 的浓度降低两到六倍，以及超声的持续时间降低两到六倍。此外，还证明了这种减少最大限度地降低了对成纤维细胞的细胞毒性，同时导致细菌浓度降低 100 到 1000 倍。
