Robertson Julia, McGoverin Cushla, White Joni R, Vanholsbeeck Frédérique, Swift Simon
Department of Molecular Medicine and Pathology, The University of Auckland, Auckland 1023, New Zealand.
The Dodd-Walls Centre for Photonic and Quantum Technologies, Auckland 1010, New Zealand.
Microorganisms. 2021 Apr 26;9(5):924. doi: 10.3390/microorganisms9050924.
Antibiotic resistance is a serious threat to public health. The empiric use of the wrong antibiotic occurs due to urgency in treatment combined with slow, culture-based diagnostic techniques. Inappropriate antibiotic choice can promote the development of antibiotic resistance. We investigated live/dead spectrometry using a fluorimeter (Optrode) as a rapid alternative to culture-based techniques through application of the LIVE/DEAD BacLight Bacterial Viability Kit. Killing was detected by the Optrode in near real-time when was treated with lytic antibiotics-ampicillin and polymyxin B-and stained with SYTO 9 and/or propidium iodide. Antibiotic concentration, bacterial growth phase, and treatment time used affected the efficacy of this detection method. Quantification methods of the lethal action and inhibitory action of the non-lytic antibiotics, ciprofloxacin and chloramphenicol, respectively, remain to be elucidated.
抗生素耐药性是对公众健康的严重威胁。由于治疗的紧迫性以及基于培养的诊断技术缓慢，会出现经验性使用错误抗生素的情况。不恰当的抗生素选择会促进抗生素耐药性的发展。我们通过应用LIVE/DEAD BacLight细菌活力试剂盒，研究了使用荧光计（Optrode）进行活/死光谱分析作为基于培养技术的快速替代方法。当用溶菌性抗生素氨苄青霉素和多粘菌素B处理并用SYTO 9和/或碘化丙啶染色时，Optrode几乎可以实时检测到细菌的杀灭情况。抗生素浓度、细菌生长阶段和使用的治疗时间会影响这种检测方法的效果。非溶菌性抗生素环丙沙星和氯霉素的致死作用和抑制作用的量化方法仍有待阐明。
