Lin Wujian, Chow Simon Kwoon Ho, Cui Can, Liu Chaoran, Wang Qianjin, Chai Senlin, Wong Ronald Man Yeung, Zhang Ning, Cheung Wing Hoi
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong Special Administrative Region of China.
Department of Rehabilitation Medicine, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
J Orthop Translat. 2024 Jun 20;47:63-73. doi: 10.1016/j.jot.2024.06.003. eCollection 2024 Jul.
The interaction between muscle and bone is shown to be clinically important but the underlying mechanisms are largely unknown. The canonical Wnt/β-catenin signaling pathway is reported to be involved in muscle-bone crosstalk, but its detailed function remains unclear. This systematic review aims to investigate and elucidate the role of the Wnt/β-catenin signaling pathways in muscle-bone crosstalk.
We conducted a literature search on the Web of Science, PubMed, EBSCO and Embase with keywords "Wnt*", "bone*" and "muscle*". A systematic review was completed according to the guideline of preferred reporting items of systematic reviews and meta-analyses (PRISMA). Data synthesis included species (human, animal or cell type used), treatments involved, outcome measures and key findings with respect to Wnts.
Seventeen papers were published from 2007 to 2021 and were extracted from a total of 1529 search results in the databases of Web of Science (468 papers), PubMed (457 papers), EBSCO (371) and Embase (233). 12 Wnt family members were investigated in the papers, including Wnt1, Wnt2, Wnt2b, Wnt3a, Wnt4, Wnt5a, Wnt8a, Wnt8b, Wnt9a, Wnt10a, Wnt10b and Wnt16. Many studies showed that muscles were able to increase or decrease osteogenesis of bone, while bone increased myogenesis of muscle through Wnt/β-catenin signaling pathways. Wnt3a, Wnt4 and Wnt10b were shown to play important roles in the crosstalk between muscle and bone.
Wnt3a, Wnt4 and Wnt10b are found to play important mediatory roles in muscle-bone crosstalk. The role of Wnt4 was mostly found to regulate muscle from the bone side. Whilst the role of Wnt10b during muscle ageing was proposed, current evidence is insufficient to clarify the specific role of Wnt/β-catenin signaling in the interplay between sarcopenia and osteoporosis. More future studies are required to investigate the exact regulatory roles of Wnts in muscle-bone crosstalk in musculoskeletal disease models such as sarcopenia and osteoporosis.
The systematic review provides an extensive overview to reveal the roles of Wnt/β-catenin signaling pathways in muscle-bone crosstalk. These results provide novel research directions to further understand the underlying mechanism of sarcopenia, osteoporosis, and their crosstalk, finally helping the future development of new therapeutic interventions.
肌肉与骨骼之间的相互作用在临床上具有重要意义，但其潜在机制在很大程度上尚不清楚。据报道，经典的Wnt/β-连环蛋白信号通路参与肌肉-骨骼的相互作用，但其详细功能仍不清楚。本系统评价旨在研究和阐明Wnt/β-连环蛋白信号通路在肌肉-骨骼相互作用中的作用。
我们在Web of Science、PubMed、EBSCO和Embase上进行了文献检索，关键词为“Wnt*”、“bone*”和“muscle*”。根据系统评价和Meta分析的首选报告项目（PRISMA）指南完成了系统评价。数据综合包括所使用的物种（人类、动物或细胞类型）、所涉及的治疗、结局指标以及关于Wnt的关键发现。
2007年至2021年共发表了17篇论文，这些论文从Web of Science（468篇论文）、PubMed（457篇论文）、EBSCO（371篇）和Embase（233篇）数据库中的1529条搜索结果中提取。论文中研究了12个Wnt家族成员，包括Wnt1、Wnt2、Wnt2b、Wnt3a、Wnt4、Wnt5a、Wnt8a、Wnt8b、Wnt9a、Wnt10a、Wnt10b和Wnt16。许多研究表明，肌肉能够通过Wnt/β-连环蛋白信号通路增加或减少骨骼的成骨作用，而骨骼则增加肌肉的肌生成作用。Wnt3a、Wnt4和Wnt10b在肌肉与骨骼的相互作用中发挥重要作用。
发现Wnt3a、Wnt4和Wnt10b在肌肉-骨骼相互作用中起重要的介导作用。大多发现Wnt4从骨骼方面调节肌肉。虽然有人提出了Wnt10b在肌肉衰老过程中的作用，但目前的证据不足以阐明Wnt/β-连环蛋白信号在肌肉减少症和骨质疏松症相互作用中的具体作用。未来需要更多的研究来调查Wnt在肌肉减少症和骨质疏松症等肌肉骨骼疾病模型中肌肉-骨骼相互作用的确切调节作用。
该系统评价提供了广泛的概述，以揭示Wnt/β-连环蛋白信号通路在肌肉-骨骼相互作用中的作用。这些结果为进一步了解肌肉减少症、骨质疏松症及其相互作用的潜在机制提供了新的研究方向，最终有助于新治疗干预措施的未来发展。
