Jinlong Hu, Yu Zhang, Ruizhi Wang, Xiaoyu Wang, Zhiming Feng, Qiangqiang Xiong, Nianbing Zhou, Yong Zhou, Haiyan Wei, Hongcheng Zhang, Jinyan Zhu
Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, 225009 China.
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009 China.
Mol Breed. 2024 Jul 11;44(7):49. doi: 10.1007/s11032-024-01486-5. eCollection 2024 Jul.
Rice blast, caused by (), is one of the most serious diseases worldwide. Developing blast-resistant rice varieties is an effective strategy to control the spread of rice blast and reduce the reliance on chemical pesticides. In this study, 477 sequenced rice germplasms from 48 countries were inoculated and assessed at the booting stage. We found that 23 germplasms exhibited high panicle blast resistance against Genome-wide association analysis (GWAS) identified 43 quantitative trait loci (QTLs) significantly associated ( < 1.0 × 10) with resistance to rice panicle blast. These QTL intervals encompass four genes (, , and /) previously reported to contribute to rice blast resistance. We selected QTLs with -Log10 (-value) greater than 6.0 or those detected in two-year replicates, amounting to 12 QTLs, for further candidate gene analysis. Three blast resistance candidate genes (, , /) were identified based on significant single nucleotide polymorphisms (SNP) distributions within annotated gene sequences across these 12 QTLs and the differential expression levels among blast-resistant varieties after 72 h of inoculation. encodes a glycine-rich protein, , an important component of plant cell walls involved in cellular stress responses and signaling. encodes a protein with unknown function (DUF953), part of the thioredoxin-like family, which is crucial for maintaining reactive oxygen species (ROS) homeostasis in vivo, named as . Lastly, / encodes a disease resistance protein, underscoring its potential importance in plant biology. By analyzing the haplotypes of these three genes, we identified favorable haplotypes for blast resistance, providing valuable genetic resources for future rice blast resistance breeding programs.
The online version contains supplementary material available at 10.1007/s11032-024-01486-5.
由（）引起的稻瘟病是全球最严重的病害之一。培育抗稻瘟病水稻品种是控制稻瘟病传播和减少对化学农药依赖的有效策略。在本研究中，对来自48个国家的477份测序水稻种质在孕穗期进行了接种和评估。我们发现23份种质对（）表现出高穗颈瘟抗性。全基因组关联分析（GWAS）确定了43个与水稻穗颈瘟抗性显著相关（ < 1.0 × 10）的数量性状位点（QTL）。这些QTL区间包含四个先前报道的有助于水稻抗稻瘟病的基因（、、和/）。我们选择-log10（-值）大于6.0或在两年重复实验中检测到的QTL，共12个QTL进行进一步的候选基因分析。基于这12个QTL注释基因序列内显著的单核苷酸多态性（SNP）分布以及接种72小时后抗稻瘟病品种间的差异表达水平，鉴定出三个抗稻瘟病候选基因（、、/）。编码一种富含甘氨酸的蛋白质，是参与细胞应激反应和信号传导的植物细胞壁的重要组成部分。编码一种功能未知的蛋白质（DUF953），属于硫氧还蛋白样家族的一部分，对维持体内活性氧（ROS）稳态至关重要，命名为。最后，/编码一种抗病蛋白，突出了其在植物生物学中的潜在重要性。通过分析这三个基因的单倍型，我们鉴定出了抗稻瘟病的有利单倍型，为未来水稻抗稻瘟病育种计划提供了有价值的遗传资源。
在线版本包含可在10.1007/s11032-024-01486-5获取的补充材料。
