Styles J A, Penman M G
Mutat Res. 1985 Nov;154(3):183-204. doi: 10.1016/0165-1110(85)90017-x.
The published results on 60 chemicals and X-rays investigated in the mouse spot test were compared with data on the same chemicals tested in the bacterial mutation assay (Ames test) and lifetime rodent bioassays. The performance of the spot test as an in vivo complementary assay to the in vitro bacterial mutagenesis test reveals that of 60 agents, 38 were positive in both systems, 6 were positive only in the spot test, 10 were positive only in the bacterial test and 6 were negative in both assays. The spot test was also considered as a predictor of carcinogenesis; 45 chemicals were carcinogenic of which 35 were detected as positive by the spot test and 3 out of 6 non-carcinogens were correctly identified as negative. If the results are regarded in sequence, i.e. that a positive result in a bacterial mutagenicity test reveals potential that may or may not be realized in vivo, then 48 chemicals were mutagenic in the bacterial mutation assay of which 38 were active in the spot test and 31 were confirmed as carcinogens in bioassays. 12 chemicals were non-mutagenic to bacteria of which 6 gave positive responses in the spot test and 5 were confirmed as carcinogens. These results provide strong evidence that the mouse coat spot test is an effective complementary test to the bacterial mutagenesis assay for the detection of genotoxic chemicals and as a confirmatory test for the identification of carcinogens. The main deficiency at present is the paucity of data from the testing of non-carcinogens. With further development and improvement of the test it is probable that the predictive performance of the assay in identifying carcinogens should improve, since many of the false negative responses may be due to inadequate testing.
将小鼠斑点试验中研究的60种化学物质和X射线的已发表结果，与在细菌突变试验（艾姆斯试验）和啮齿动物终身生物测定中对相同化学物质进行测试得到的数据进行了比较。斑点试验作为体外细菌诱变试验的体内补充试验的表现表明，在60种试剂中，38种在两个系统中均呈阳性，6种仅在斑点试验中呈阳性，10种仅在细菌试验中呈阳性，6种在两种试验中均为阴性。斑点试验也被视为致癌作用的预测指标；45种化学物质具有致癌性，其中35种通过斑点试验检测为阳性，6种非致癌物中有3种被正确鉴定为阴性。如果按顺序看待这些结果，即细菌诱变性试验中的阳性结果表明体内可能实现或可能未实现的潜力，那么在细菌突变试验中有48种化学物质具有诱变性，其中38种在斑点试验中具有活性，31种在生物测定中被确认为致癌物。12种化学物质对细菌无诱变性，其中6种在斑点试验中给出阳性反应，5种被确认为致癌物。这些结果提供了强有力的证据，表明小鼠皮毛斑点试验是检测遗传毒性化学物质的细菌诱变试验的有效补充试验，也是鉴定致癌物的确认试验。目前的主要不足是来自非致癌物测试的数据匮乏。随着该试验的进一步发展和改进，该试验在鉴定致癌物方面的预测性能可能会提高，因为许多假阴性反应可能是由于测试不足所致。
