Chronwall B M, DiMaggio D A, Massari V J, Pickel V M, Ruggiero D A, O'Donohue T L
Neuroscience. 1985 Aug;15(4):1159-81. doi: 10.1016/0306-4522(85)90260-x.
The distribution of neuropeptide Y in the central nervous system of adult male rats was investigated using indirect immunofluorescence, the peroxidase-antiperoxidase technique and by radioimmunoassay of microdissected brain regions. The different methods were in good agreement and showed that neuropeptide Y had a widespread distribution and was present in extremely high concentrations. The highest concentrations of neuropeptide Y were found in the paraventricular hypothalamic nucleus and hypothalamic arcuate nucleus, which also contained the highest density of immunoreactive fibers and numbers of perikarya, respectively. The suprachiasmatic nucleus, median eminence, dorsomedial hypothalamic nucleus and paraventricular thalamic nucleus showed high concentrations as well as high densities of fibers. Moderate concentrations were found in the bed nucleus of the stria terminalis, although a high density of fibers was found. Areas with moderate concentrations and densities of fibers were the medial preoptic area, anterior hypothalamic area, periventricular nucleus, posterior hypothalamus and the medial amygdaloid nucleus. The nucleus of the solitary tract contained a low concentration of neuropeptide Y although a high number of immunoreactive perikarya was found in colchicine-treated rats. Low concentrations were also measured in the cerebral cortex, yet relatively high numbers of cell bodies and fibers were found dispersed through the cortex. The extremely high concentrations and widespread distribution of neuropeptide Y in the central nervous system suggests a number of important physiological roles for this neurotransmitter candidate.
采用间接免疫荧光法、过氧化物酶-抗过氧化物酶技术以及对显微切割的脑区进行放射免疫测定，对成年雄性大鼠中枢神经系统中神经肽Y的分布进行了研究。不同方法所得结果高度一致，表明神经肽Y分布广泛且浓度极高。神经肽Y浓度最高的部位是下丘脑室旁核和下丘脑弓状核，分别也是免疫反应性纤维密度最高和核周体数量最多的部位。视交叉上核、正中隆起、下丘脑背内侧核和丘脑室旁核也显示出高浓度以及高密度的纤维。终纹床核中虽纤维密度高，但神经肽Y浓度中等。纤维浓度和密度中等的区域有内侧视前区、下丘脑前区、室周核、下丘脑后部和杏仁内侧核。孤束核中神经肽Y浓度低，不过在秋水仙碱处理的大鼠中发现了大量免疫反应性核周体。大脑皮质中也检测到低浓度，但发现有相对较多的细胞体和纤维分散在皮质中。神经肽Y在中枢神经系统中浓度极高且分布广泛，这表明这种神经递质候选物具有许多重要的生理作用。
