Institute for Applied and Environmental Microbiology, College of Life Science, Inner Mongolia Agricultural University, Huhhot 010018, PR China.
Int J Syst Evol Microbiol. 2020 Mar;70(3):2089-2095. doi: 10.1099/ijsem.0.004020.
A pink-pigmented, Gram-negative, rod-shaped, obligate aerobic bacterial strain, MIMD6, was isolated from biological soil crusts in PR China. Cells grew at 20-37 °C (optimum, 30 °C), at pH 6-8 (optimum, pH 7) and with 0-1 % (w/v) NaCl (optimum, 0 %). Strain MIMD6 could use methanol or formate as a sole carbon source to grow, and carried methanol dehydrogenase genes and , supporting its methylotrophic metabolism. The respiratory quinone was ubiquinone Q-10, the major fatty acids were Cω7 (87.3 %), and the major polar lipids were diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unknown aminolipid and one unidentified glycolipid. The results of phylogenetic analyses based on the sequences of the 16S rRNA gene, seven housekeeping genes (, , , , , and ) and methanol dehydrogenase genes indicated that strain MIMD6 formed a phylogenetic linage with members of the genus . Strain MIMD6 was most closely related to DSM 17168 and LMG 21967 with 16S rRNA gene sequence similarities of 95.7 and 95.2 %, respectively. The genomic DNA G+C content calculated via draft genome sequencing was 73.0 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain MIMD6 and the type strains of other species were 70.7-82.0 and 24.6-30.0 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain MIMD6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MIMD6 (=KCTC 52305=MCCC 1K01311).
一株从中国 PR 生物土壤结皮中分离到的粉红着色、革兰氏阴性、杆状、专性需氧细菌菌株 MIMD6。细胞在 20-37°C（最适温度 30°C）、pH 6-8（最适 pH 7）和 0-1%（w/v）NaCl（最适 0%）下生长。菌株 MIMD6 可以甲醇或甲酸盐作为唯一碳源生长，并携带甲醇脱氢酶基因和，支持其甲基营养代谢。呼吸醌为泛醌 Q-10，主要脂肪酸为 Cω7（87.3%），主要极性脂为双磷脂酰甘油、磷脂单甲基乙醇胺、磷脂乙醇胺、磷脂甘油、磷脂胆碱、一种未知的氨基脂和一种未鉴定的糖脂。基于 16S rRNA 基因、7 个看家基因（、、、、、和）和甲醇脱氢酶基因序列的系统发育分析结果表明，菌株 MIMD6 与属内的成员形成了一个系统发育分支。菌株 MIMD6 与 DSM 17168 和 LMG 21967 的 16S rRNA 基因序列相似性最高，分别为 95.7%和 95.2%。通过草案基因组测序计算的基因组 DNA G+C 含量为 73.0mol%。与其他种的模式菌株相比，菌株 MIMD6 的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 70.7-82.0%和 24.6-30.0%。基于表型、化学分类和系统发育特征，菌株 MIMD6 代表了属的一个新种，建议命名为 sp. nov.。模式菌株为 MIMD6（=KCTC 52305=MCCC 1K01311）。
