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反刍兽考德里氏体的体外培养。

In vitro cultivation of Cowdria ruminantium.

作者信息

Bezuidenhout J D, Paterson C L, Barnard B J

出版信息

Onderstepoort J Vet Res. 1985 Jun;52(2):113-20.

PMID:3900854
Abstract

Cowdria ruminantium was cultivated in a calf endothelial cell line after the cells had been irradiated at 45 & 90 GY. Another experiment in which the inoculum and non-irradiated cells were centrifuged together also yielded positive results. In some irradiated cultures, colonies of organisms could be demonstrated microscopically up to 70 days after the cultures had been inoculated with infected tick stabilate. The infectivity of cultures, even after 4 passages and 88 days post-inoculation, was demonstrated by their intravenous injection in sheep.

摘要

在小牛内皮细胞系中,于细胞经45和90戈瑞照射后培养反刍兽考德里氏体。另一项将接种物与未照射细胞一起离心的实验也得到了阳性结果。在一些经照射的培养物中,在接种感染蜱稳定期物后长达70天,可在显微镜下观察到微生物菌落。通过将培养物静脉注射到绵羊体内,证实了即使在传代4次和接种后88天,培养物仍具有感染性。

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引用本文的文献

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