Sahu S P
Am J Vet Res. 1986 Jun;47(6):1253-7.
Fluorescent antibody tests, Giemsa stain, and electron microscopy were used to detect colonies of Cowdria ruminantium in in vitro-cultured macrophages and buffy coats from heartwater-infected cattle, sheep, and goats. Antibodies were obtained from C ruminantium-infected cattle, sheep, and goats treated with a small dose of oxytetracycline HCl. Cowdria ruminantium elementary bodies were small-coccus forms (0.14 micron) and large-coccus forms (0.22 micron to 0.6 micron). The size of inclusion bodies varied from 1.5 micron to 2 micron. Inclusion bodies and elementary bodies were observed in the cytoplasm of macrophages and neutrophils.
采用荧光抗体试验、吉姆萨染色和电子显微镜检测法，对来自感染心水病的牛、羊和山羊的体外培养巨噬细胞及血沉棕黄层中的反刍兽考德里氏体菌落进行检测。抗体取自经小剂量盐酸土霉素治疗的感染反刍兽考德里氏体的牛、羊和山羊。反刍兽考德里氏体的原体呈小球菌形态（0.14微米）和大球菌形态（0.22微米至0.6微米）。包涵体大小在1.5微米至2微米之间。在巨噬细胞和中性粒细胞的细胞质中观察到了包涵体和原体。
