Prozesky L
Veterinary Research Institute, Onderstepoort.
Onderstepoort J Vet Res. 1987 Sep;54(3):277-9.
The artificial transmission of Cowdria ruminantium with infected blood, organ homogenates, peritoneal macrophages, tick stabilate and tissue culture cells is discussed. Organ homogenates prepared from the myocardium, spleen, kidneys and liver of diseased animals are commonly used to infect mice. The efficacy of organ homogenates as a source of C. ruminantium depends on factors such as the route of inoculation and the heartwater isolate used. Heartwater is artificially transmitted with infected tick stabilate, haemocytes, rectal ampules and hypodermal homogenates. The infectivity of saliva collected from Amblyomma hebraeum female ticks was very low compared to the ground-up suspensions prepared from the same group of ticks.
本文讨论了用感染血液、器官匀浆、腹腔巨噬细胞、蜱传稳定材料和组织培养细胞人工传播反刍兽考德里氏体的情况。通常用患病动物的心肌、脾脏、肾脏和肝脏制备的器官匀浆来感染小鼠。器官匀浆作为反刍兽考德里氏体来源的效力取决于接种途径和所用的牛心水分离株等因素。牛心水通过感染的蜱传稳定材料、血细胞、直肠安瓿和皮下匀浆进行人工传播。与同一组蜱制备的研磨悬浮液相比，从希伯来花蜱雌蜱采集的唾液传染性非常低。
