Hutter K J, Eipel H E
J Gen Microbiol. 1979 Aug;113(2):369-75. doi: 10.1099/00221287-113-2-369.
Recent improvements in the optics and electronics of flow cytometry systems, as well as in staining techniques, permit the assay of such minute cellular constituents as the DNA and protein contents of micro-organisms. To assess the usefulness of this technique, DNA and protein content distributions were determined in Escherichia coli, Lactobacillus brevis, Lactobacillus casei, Chlorella kessleri 8k, Saccharomyces cerevisiae, Candida utilis, Schizosaccharomyces pombe and Euglena gracilis. Investigations of the DNA content distributions of polyploid strains of Saccharomyces cerevisiae indicated that the method can be used to determine ploidy. The rapidity of flow cytometry measurements allows accurate determinations in large populations.
流式细胞术系统的光学和电子学技术以及染色技术最近取得的进展，使得能够检测诸如微生物的DNA和蛋白质含量等微小细胞成分。为了评估该技术的实用性，我们测定了大肠杆菌、短乳杆菌、干酪乳杆菌、凯氏小球藻8k、酿酒酵母、产朊假丝酵母、粟酒裂殖酵母和纤细裸藻的DNA和蛋白质含量分布。对酿酒酵母多倍体菌株DNA含量分布的研究表明，该方法可用于确定倍性。流式细胞术测量的快速性使得能够对大量群体进行准确测定。
