Department of Drug Science and Technology and NIS - Centre, University of Turin, Via Pietro Giuria 9, Turin, 10125, Italy.
Country College of Chemistry and Chemical Engineering, Chemistry and Chemical Engineering Guangdong Province Laboratory, Shantou University, Daxue Road 243, Shantou, 515063, China.
AAPS PharmSciTech. 2024 Jul 15;25(6):165. doi: 10.1208/s12249-024-02872-0.
CaCO nanoparticles (nano-CaCO) as nano-templates were prepared using CaCl and NaCO solutions under controlled sonication (19.5 kHz). Using the same ultrasonic device, subsequently, hollow mesoporous silica nanoparticles (HMSNs) were obtained by the hard template of nano-CaCO. HMSNs were selected as carriers for the antifungal drug voriconazole (VOR) loading to overcome poor water solubility. Three-dimensional CaCO nanosheets HMSNs were obtained under gentle sonication. Three-dimensional CaCO nanosheets of 24.5 nm (hydrodynamic diameter) were obtained under 17.6 W for 3 min. HMSNs were synthesized by double-template method with nano-CaCO as the hard template. Transmission electron microscopy measurements showed that the prepared HMSNs possess hollow structures with particle size between 110 and 120 nm. Nitrogen physisorption at -196 °C revealed that the HMSNs had high surface area (401.57 m/g), high pore volume (0.11 cm/g), and uniform pore size (2.22 nm) that facilitated the effective encapsulation of VOR in the HMSNs. The loading capacity of VOR (wt%) on the HMSNs was 7.96%, and the total VOR release amount of VOR-HMSNs material was 71.40% at 480 min. The kinetic model confirmed that the release mechanism of HMSNs nanoparticles followed Fickian diffusion at pH = 7.4 and 37 °C. Moreover, the cumulative VOR release at 42 °C (86.05%) was higher than that at 37 °C (71.40%). The cumulative release amount of VOR from the VOR-HMSNs material was 92.37% at pH = 5.8 at the same temperature. Both nano-CaCO templates and HMSNs were prepared by sonication at 19.5 kHz. The as-prepared HMSNs can effectively encapsulate VOR and released drug by Fickian diffusion.
碳酸钙纳米粒子（纳米-CaCO）作为纳米模板，使用 CaCl 和 NaCO 溶液在超声控制下（19.5 kHz）制备。随后，使用相同的超声设备，通过纳米-CaCO 的硬模板，得到中空介孔硅纳米粒子（HMSNs）。选择 HMSNs 作为抗真菌药物伏立康唑（VOR）负载的载体，以克服其较差的水溶性。在温和的超声下得到了三维 CaCO 纳米片 HMSNs。在 17.6 W 下超声 3 分钟，得到了 24.5nm（水动力学直径）的三维 CaCO 纳米片。通过双模板法用纳米-CaCO 作为硬模板合成 HMSNs。透射电子显微镜测量表明，所制备的 HMSNs 具有空心结构，粒径在 110nm 至 120nm 之间。-196°C 下的氮气物理吸附表明，HMSNs 具有高表面积（401.57m/g）、高孔体积（0.11cm/g）和均匀的孔径（2.22nm），有利于 VOR 有效包封在 HMSNs 中。VOR（wt%）在 HMSNs 上的负载量为 7.96%，VOR-HMSNs 材料的总 VOR 释放量在 480min 时达到 71.40%。动力学模型证实，在 pH=7.4 和 37°C 时，HMSNs 纳米粒子的释放机制遵循菲克扩散。此外，在 42°C（86.05%）时的累积 VOR 释放量高于在 37°C（71.40%）时的释放量。在相同温度下，VOR-HMSNs 材料的 VOR 累积释放量在 pH=5.8 时达到 92.37%。纳米-CaCO 模板和 HMSNs 均通过 19.5kHz 的超声制备。所制备的 HMSNs 可以有效地包封 VOR，并通过菲克扩散释放药物。
