ICAR-Sugarcane Breeding Institute, Coimbatore, Tamil Nadu, 641007, India.
AgriGenome Labs, Infopark-Smart City Short Rd, Kochi, Kerala, 682030, India.
Plant Cell Rep. 2021 May;40(5):851-870. doi: 10.1007/s00299-021-02682-9. Epub 2021 Apr 5.
Sugarcane microRNAs specifically involved during compatible and incompatible interactions with red rot pathogen Colletotrichum falcatum were identified. We have identified how the miRNAs regulate their gene targets and elaborated evidently on the underlying molecular mechanism of sugarcane defense response to C. falcatum for the first time. Resistance against the fungal pathogen Colletotrichum falcatum causing red rot is one of the most desirable traits for sustainable crop cultivation in sugarcane. To gain new insight into the host defense mechanism against C. falcatum, we studied the role of sugarcane microRNAs during compatible and incompatible interactions by adopting the NGS platform. We have sequenced a total of 80 miRNA families that comprised 980 miRNAs, and the putative targets of the miRNAs include transcription factors, membrane-bound proteins, glutamate receptor proteins, lignin biosynthesis proteins, signaling cascade proteins, transporter proteins, mitochondrial proteins, ER proteins, defense-related, stress response proteins, translational regulation proteins, cell proliferation, and ubiquitination proteins. Further, qRT-PCR analyses of 8 differentially regulated miRNAs and 26 gene transcript targets expression indicated that these miRNAs have a regulatory effect on the expression of respective target genes in most of the cases. Also, the results suggest that certain miRNA regulates many target genes that are involved in inciting early responses to the pathogen infection, signaling pathways, endoplasmic reticulum stress, and resistance gene activation through feedback response from various cellular processes during the compatible and incompatible interaction with the red rot pathogen C. falcatum. The present study revealed the role of sugarcane miRNAs and their target genes during sugarcane-C. falcatum interaction and provided new insight into the miRNA-mediated defense mechanism in sugarcane for the first time.
在与红腐病菌 Colletotrichum falcatum 互作的过程中，鉴定出了特定参与的甘蔗 microRNA。我们已经确定了 microRNA 如何调控其基因靶标，并首次详细阐述了甘蔗防御反应对 C. falcatum 的潜在分子机制。对引起红腐病的真菌病原体 Colletotrichum falcatum 的抗性是甘蔗可持续种植最理想的性状之一。为了深入了解甘蔗对 C. falcatum 的防御机制，我们通过采用 NGS 平台研究了 microRNA 在亲和和非亲和互作过程中的作用。我们总共测序了 80 个 miRNA 家族，包含 980 个 miRNA，miRNA 的假定靶标包括转录因子、膜结合蛋白、谷氨酸受体蛋白、木质素生物合成蛋白、信号级联蛋白、转运蛋白、线粒体蛋白、内质网蛋白、防御相关蛋白、应激反应蛋白、翻译调控蛋白、细胞增殖和泛素化蛋白。此外，对 8 个差异调控 miRNA 和 26 个基因转录靶标表达的 qRT-PCR 分析表明，在大多数情况下，这些 miRNA 对各自靶基因的表达具有调控作用。此外，结果表明，某些 miRNA 调控许多靶基因，这些靶基因参与激发对病原体感染的早期反应、信号通路、内质网应激和抗性基因激活，通过与红腐病菌 C. falcatum 的亲和和非亲和互作过程中的各种细胞过程的反馈反应。本研究揭示了甘蔗 microRNA 及其靶基因在甘蔗- C. falcatum 互作过程中的作用，并首次为甘蔗提供了 miRNA 介导的防御机制的新见解。
