评价泰国本土水果的植物化学成分谱、抗氧化和抗糖尿病活性。

Evaluation of phytochemical profile, and antioxidant, antidiabetic activities of indigenous Thai fruits.

机构信息

Department of Botany, Kasetsart University, Bangkok, Thailand.

Department of Medical Science, Ministry of Public Health, Medicinal Plant Research Institute, Nonthaburi, Thailand.

出版信息

PeerJ. 2024 Jul 12;12:e17681. doi: 10.7717/peerj.17681. eCollection 2024.

DOI:10.7717/peerj.17681

PMID:39011385

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11249001/

Abstract

BACKGROUND

This research aims to explore the phenolics identification, phenolics quantification, antioxidant and potential biofunctional properties of lesser-known Thai fruits and their potency to treat type 2 diabetes mellitus (T2DM). Including, and were analyzed by their phenolic and flavonoid content. These fruits have received limited scientific attention, prompting an investigation into their health benefits, particularly their relevance to diabetes management.

METHODS

The study utilized methanolic crude extracts to measure phenolic and flavonoid levels. Additionally, UHPLC-DAD was utilized to identify and quantify phenolics. The methanolic extracts were assessed for antioxidant and antidiabetic abilities, including α-glucosidase and α-amylase inhibition.

RESULTS AND CONCLUSION

The study highlighted as a rich source of phenolic (980.42 ± 0.89 mg GAE/g and flavonoid (3.55 ± 0.02 mg QE/g) compounds with strong antioxidant activity (IC by DPPH; 3.00 ± 0.01 µg/ml, IC by ABTS; 40 ± 0.01 µg/ml, FRAP; 898.63 ± 0.02 mg TE/ml). Additionally, exhibited promising antidiabetic effects ( IC; 0.13 ± 0.01 mg/ml for α-glucosidase inhibition, 3.91 ± 0.05 mg/ml for α-amylase inhibition), compared to Acarbose (IC; 0.86 ± 0.01 mg/ml for α-glucosidase inhibition, 0.39 ± 0.05 mg/ml for α-amylase inhibition). Remarkably, compounds like catechins, gallic acid, kaempferol, and ellagic acid were identified in various quantities.This study suggests that these fruits, packed with phenolics, hold the potential to be included in an anti-diabetic diet and even pharmaceutical applications due to their health-promoting properties.

摘要

背景

本研究旨在探索鲜为人知的泰国水果中的酚类物质鉴定、定量分析、抗氧化和潜在的生物功能特性，以及它们治疗 2 型糖尿病（T2DM）的潜力。包括、和，通过其酚类和类黄酮含量进行分析。这些水果受到的科学关注有限，因此我们研究了它们的健康益处，特别是它们与糖尿病管理的相关性。

方法

本研究使用甲醇粗提取物来测量酚类和类黄酮的含量。此外，还使用 UHPLC-DAD 来鉴定和定量酚类物质。评估了甲醇提取物的抗氧化和抗糖尿病能力，包括α-葡萄糖苷酶和α-淀粉酶抑制作用。

结果与结论

研究结果表明，是酚类（980.42 ± 0.89 mg GAE/g）和类黄酮（3.55 ± 0.02 mg QE/g）化合物的丰富来源，具有很强的抗氧化活性（DPPH 的 IC 为 3.00 ± 0.01 µg/ml，ABTS 的 IC 为 40 ± 0.01 µg/ml，FRAP 的 IC 为 898.63 ± 0.02 mg TE/ml）。此外，表现出有希望的抗糖尿病作用（α-葡萄糖苷酶抑制的 IC 为 0.13 ± 0.01 mg/ml，α-淀粉酶抑制的 IC 为 3.91 ± 0.05 mg/ml），与阿卡波糖（α-葡萄糖苷酶抑制的 IC 为 0.86 ± 0.01 mg/ml，α-淀粉酶抑制的 IC 为 0.39 ± 0.05 mg/ml）相比。值得注意的是，儿茶素、没食子酸、山奈酚和鞣花酸等化合物以不同的量被鉴定出来。本研究表明，这些富含酚类化合物的水果具有作为抗糖尿病饮食的一部分，甚至是药物应用的潜力，因为它们具有促进健康的特性。