Ma Liqing, Jian Yingna, Wang Guanghua, Cai Qigang, Wang Geping, Li Xiuping, Zhang Xueyong, Karanis Panagiotis
Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China.
Medical Faculty and University Hospital Cologne, University of Cologne, Cologne, Cologne, Germany.
Iran J Parasitol. 2024 Apr-Jun;19(2):162-170. doi: 10.18502/ijpa.v19i2.15852.
and are well-known important intestinal zoonotic pathogens that can infect various hosts and cause diarrhoeal diseases. We aimed to determine the epidemiological prevalence and molecular characterization of and species in Himalayan marmot (, class Marmota) in the Qinghai Tibetan Plateau Area of Qinghai Province, Northwest China.
Overall, 243 Himalayan marmot fecal samples were collected in 2017 and in 2019 and a two-step nested PCR technique was performed to amplify the fragments of the SSU rRNA gene of and 18S ribosomal RNA gene of . Molecular characterization of was performed with the primary primers NDIAGF2 and N-DIAGR2, the secondary primers CPB-DIAGF and CPB-DIAGR. Similarly, molecular characterization of was used the first primers Gia2029 and Gia2150c, the secondary primers RH11 and RH4. The positive PCR products were sequenced and the sequences were processed by Clustal Omega and BLAST. Phylogenetic analysis was achieved by NJ method in MEGA.
The infection rate of spp. and . was 4.9% (12/243) and 0.8% (2/243) in . , respectively. spp. are characterized as novel genotypes marmot genotype I (n=3) and marmot genotype II (n=9); . assemblage A (n=2) was found in . .
This is the first report of spp. and . infections in . in Qinghai of Northwest China. The results indicate the existence of species and . infections that may have a potential public health significance.
[病原体名称1]和[病原体名称2]是众所周知的重要肠道人畜共患病原体，可感染多种宿主并引起腹泻疾病。我们旨在确定中国西北青海省青藏高原地区喜马拉雅旱獭（哺乳纲旱獭属）中[病原体名称1]和[病原体名称2]物种的流行病学流行率和分子特征。
总体而言，2017年和2019年共收集了243份喜马拉雅旱獭粪便样本，并采用两步巢式PCR技术扩增[病原体名称1]的SSU rRNA基因片段和[病原体名称2]的18S核糖体RNA基因片段。[病原体名称1]的分子特征分析使用一级引物NDIAGF2和N - DIAGR2，二级引物CPB - DIAGF和CPB - DIAGR。同样，[病原体名称2]的分子特征分析使用一级引物Gia2029和Gia2150c，二级引物RH11和RH4。对阳性PCR产物进行测序，并通过Clustal Omega和BLAST对序列进行处理。在MEGA中通过邻接法进行系统发育分析。
在喜马拉雅旱獭中，[病原体名称1]属和[病原体名称2]的感染率分别为4.9%（12/243）和0.8%（2/243）。[病原体名称1]属被鉴定为新基因型，喜马拉雅旱獭基因型I（n = 3）和喜马拉雅旱獭基因型II（n = 9）；在喜马拉雅旱獭中发现了[病原体名称2]组合A（n = 2）。
这是中国西北青海喜马拉雅旱獭中[病原体名称1]属和[病原体名称2]感染的首次报告。结果表明存在[病原体名称1]物种和[病原体名称2]感染，可能具有潜在的公共卫生意义。
