Yazdani Amin, Khamesi Narges, Keyhani Alireza, Nasibi Saeid, Mohammadi Mohammad Ali, Mousavi Seyed Mohammad, Derakhshani Ali, Fasihi Harandi Majid
Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Iran J Parasitol. 2024 Apr-Jun;19(2):131-139. doi: 10.18502/ijpa.v19i2.15849.
We aimed to evaluate the differential expression of and genes in the protoscoleces, germinal layer, the neck, and the sucker regions of adult .
The study was conducted in 2018 at the Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran. In the present study protoscoleces were cultured in a di-phasic medium to obtain strobilated worms. The strobilated worms were harvested and using a sterile razor blade, the neck region was separated. In the molecular study the neck sections were compared with the tissues derived from the suckers from the same worm. The primers were specifically designed for RT-qPCR on and . The germinative cells were isolated from the cyst germinal layer and cultured in DMEM for further molecular studies. The Immunohisto-chemical profile was designed to explore the nature of protein in the strobilated worms. Differences between and within groups were statistically assessed relative to the protoscoleces.
An increasing gene expressions were found in sucker, neck, cells and germinal layer in comparison to the protoscoleces. The expression of ago gene was decreased in sucker, cell and germinal layer, and increased in the neck region in comparison to the protoscoleces. The results showed that both genes were expressed in all developmental stages of .
and genes were differentially expressed at different developmental stages of and may contribute to differentiation of the parasite.
我们旨在评估[基因名称1]和[基因名称2]基因在成虫原头节、生发层、颈部和吸盘区域的差异表达。
该研究于2018年在伊朗克尔曼医科大学的包虫病研究中心进行。在本研究中，将原头节培养在双相培养基中以获得节片化的虫体。收获节片化的虫体，并用无菌剃须刀片分离颈部区域。在分子研究中，将颈部切片与来自同一条虫体吸盘的组织进行比较。针对[基因名称1]和[基因名称2]专门设计了用于RT-qPCR的引物。从囊肿生发层分离出生殖细胞，并在DMEM中培养以进行进一步的分子研究。设计免疫组织化学分析以探究节片化虫体中[蛋白名称]的性质。相对于原头节，对组间和组内差异进行统计学评估。
与原头节相比，在吸盘、颈部、细胞和生发层中发现[基因名称1]基因表达增加。与原头节相比，[基因名称2]基因在吸盘、细胞和生发层中的表达降低，而在颈部区域增加。结果表明这两个基因在[虫体名称]的所有发育阶段均有表达。
[基因名称1]和[基因名称2]基因在[虫体名称]的不同发育阶段存在差异表达，可能有助于寄生虫的分化。
