Yildiz Rahmi, Aydemir Selahattin, Halidi Ahmet Galip, Unlu Ahmet Hakan, Yilmaz Hasan
Division of Veterinary, Vocational School of Gevas, University of Van Yuzuncu Yil, Van, Türkiye.
Department of Parasitology, Faculty of Medicine, University of Van Yuzuncu Yil, Van, Türkiye.
Iran J Parasitol. 2024 Apr-Jun;19(2):247-254. doi: 10.18502/ijpa.v19i2.15860.
We aimed to determine the common genotypes in Ağrı, Türkiye and to obtain information on the transmission of this parasite.
Cystic echinococcosis samples from 100 slaughtered cattle and 100 slaughtered sheep and faecal samples from 200 stray dogs were included in 2021. Collected cyst fluid samples and faces were examined microscopically. DNA was isolated from the germinal membrane of the cysts and from the parasite eggs in the stool samples. The mitochondrial gene region of the parasite was amplified by PCR. Genotypes were determined using the Basic Local Alignment Search Tool (BLAST) after sequence analysis of PCR amplicons.
The highest percentage of cysts was found in the lungs of sheep and the liver of cattle. In addition, 75% of sheep cysts and 25.6% of cattle cysts were fertile. Taenia spp./ spp. eggs were found in 6% of the faeces of 200 dogs examined microscopically. eggs were detected in 4 out of 50 stool samples analysed by PCR. All samples analysed by sequence analysis were identified as s.s. G1 genotype. Sequence comparison revealed revealed one or more-point mutations in different regions of the five samples.
s.s. G1 genotype, known as sheep strain, is common in the Ağrı, Türkiye. The controlled slaughter of livestock, especially sheep, and the avoidance of feeding hydatid cyst organs to dogs, together with public education, were necessary to prevent the spread of the disease.
我们旨在确定土耳其阿格里省的常见基因型，并获取有关这种寄生虫传播的信息。
2021年纳入了100头屠宰牛和100只屠宰羊的囊性棘球蚴病样本以及200只流浪狗的粪便样本。对采集的囊液样本和粪便进行显微镜检查。从囊肿的生发膜和粪便样本中的寄生虫卵中分离DNA。通过聚合酶链反应（PCR）扩增寄生虫的线粒体基因区域。在对PCR扩增产物进行序列分析后，使用基本局部比对搜索工具（BLAST）确定基因型。
在绵羊的肺部和牛的肝脏中发现囊肿的比例最高。此外，75%的绵羊囊肿和25.6%的牛囊肿是有繁殖力的。在显微镜检查的200只狗的粪便中，6%发现了带绦虫属/种的卵。在通过PCR分析的50份粪便样本中，有4份检测到卵。通过序列分析的所有样本均被鉴定为细粒棘球绦虫G1基因型。序列比较显示，五个样本的不同区域存在一个或多个点突变。
被称为绵羊株的细粒棘球绦虫G1基因型在土耳其阿格里省很常见。为防止疾病传播，有必要对家畜尤其是绵羊进行控制性屠宰，避免用包虫囊肿器官喂狗，并开展公众教育。
