Faruk M O, Siddiki A M A M Z, Masuduzzaman M, Chowdhury S, Hossain M A
Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225, Bangladesh.
Trop Biomed. 2017 Dec 1;34(4):831-840.
The tapeworm Echinococcus granulosus causes cystic echinococcosis (CE) in human and many domestic animals worldwide including Bangladesh. The parasite has significant public health importance in the country and no in-depth study has been conducted to determine this cestode in either human or animals. The aim of present study was to evaluate genotype of E. granulosus isolated from domestic goats reared in Chittagong, Bangladesh using DNA based tools. Partial gene fragment of 12S rRNA gene and Cytochrome oxidase 1 gene were accomplished by PCR followed by sequencing and phylogenetic analyses. A total of 19 hydatid cyst samples were collected from 385 goats from several local slaughterhouses located in Chittagong. The rates of fertile hydatid cysts were found as high as 57.89% while remaining cysts were found non-viable and sterile. Genomic DNA was extracted from germinal membrane and/or protoscolices for PCR assay. Sequence similarity based on BLAST search revealed variable prevalence of E. granulosus genotypes such as G1 (68.42%) and G1/G3 complex (31.58%) which is reported for the first time in the country. This result indicates common sheep strain G1 is the dominant subtype of E. granulosus in this region. The study generated six sequences of which four were aligned with G1 common sheep strain and two were aligned with G3 strain (commonly referred as Buffalo strain). Phylogenetic analysis of 12S rRNA gene and Cytochrome oxidase 1 gene also indicated that common sheep strain (G1) and Buffalo strain (G3) are circulating among domestic goats in Chittagong region of Bangladesh.
细粒棘球绦虫可在包括孟加拉国在内的全球许多国家的人类和多种家畜中引发囊型包虫病（CE）。该寄生虫在该国具有重大的公共卫生意义，然而尚未开展深入研究以确定人类或动物体内的这种绦虫。本研究的目的是利用基于DNA的工具评估从孟加拉国吉大港饲养的家山羊中分离出的细粒棘球绦虫的基因型。通过聚合酶链反应（PCR）扩增12S核糖体RNA（rRNA）基因和细胞色素氧化酶1基因的部分基因片段，随后进行测序和系统发育分析。从吉大港几个当地屠宰场的385只山羊中总共收集了19个包虫囊肿样本。发现可育包虫囊肿的比例高达57.89%，而其余囊肿则无活力且不育。从生发膜和/或原头节中提取基因组DNA用于PCR检测。基于BLAST搜索的序列相似性显示细粒棘球绦虫基因型的不同流行率，如G1（68.42%）和G1/G3复合体（31.58%），这是该国首次报道。该结果表明常见的绵羊株G1是该地区细粒棘球绦虫的主要亚型。该研究产生了六个序列，其中四个与G1常见绵羊株比对，两个与G3株（通常称为水牛株）比对。12S rRNA基因和细胞色素氧化酶1基因的系统发育分析也表明常见绵羊株（G1）和水牛株（G3）在孟加拉国吉大港地区的家山羊中传播。
