Nie L, Nusantara A C, Damle V G, Sharmin R, Evans E P P, Hemelaar S R, van der Laan K J, Li R, Perona Martinez F P, Vedelaar T, Chipaux M, Schirhagl R
University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering, A. Deusinglaan 1, 9713 AV Groningen, Netherlands.
Institute of Physics, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
Sci Adv. 2021 May 19;7(21). doi: 10.1126/sciadv.abf0573. Print 2021 May.
Free radicals play a vital role in all kinds of biological processes including immune responses. However, free radicals have short lifetimes and are highly reactive, making them difficult to measure using current methods. Here, we demonstrate that relaxometry measurement, or T1, inherited from the field of diamond magnetometry can be used to detect free radicals in living cells with subcellular resolution. This quantum sensing technique is based on defects in diamond, which convert a magnetic signal into an optical signal, allowing nanoscale magnetic resonance measurements. We functionalized fluorescent nanodiamonds (FNDs) to target single mitochondria within macrophage cells to detect the metabolic activity. In addition, we performed measurements on single isolated mitochondria. We were able to detect free radicals generated by individual mitochondria in either living cells or isolated mitochondria after stimulation or inhibition.
自由基在包括免疫反应在内的各种生物过程中起着至关重要的作用。然而，自由基寿命短且反应性高，使得用现有方法测量它们很困难。在这里，我们证明，从金刚石磁力测量领域继承而来的弛豫测量法，即T1，可以用于以亚细胞分辨率检测活细胞中的自由基。这种量子传感技术基于金刚石中的缺陷，这些缺陷将磁信号转换为光信号，从而实现纳米级磁共振测量。我们对荧光纳米金刚石（FND）进行功能化处理，使其靶向巨噬细胞内的单个线粒体，以检测代谢活性。此外，我们对单个分离的线粒体进行了测量。我们能够检测到在刺激或抑制后，活细胞或分离线粒体中单个线粒体产生的自由基。
Zotero 插件
