Swift Current Research and Development Centre, Agriculture and Agri-Food Canada, Swift Current, Saskatchewan, Canada.
Aquatic and Crop Resource Development, National Research Council Canada, Ottawa, Canada.
Phytopathology. 2024 Nov;114(11):2401-2411. doi: 10.1094/PHYTO-09-23-0348-R. Epub 2024 Nov 21.
Durum wheat () is threatened by the appearance of new virulent races of leaf rust, caused by , in recent years. This study was conducted to determine the leaf rust resistance in a modern Canadian durum cultivar, Strongfield. Six populations derived from crosses of Strongfield with six tetraploid wheat lines, respectively, were tested at the seedling plant stage with different races. Two of the populations were evaluated for adult plant leaf rust infection in Canada and Mexico. A stepwise regression joint linkage quantitative trait locus (QTL) mapping and analysis by MapQTL were performed. Strongfield contributed the majority of QTLs detected, contributing seven QTLs detected in field tests and eight QTLs conditioning seedling resistance. A 1B QTL, , from Strongfield had a significant effect in both Canadian and Mexican field tests and corresponded with /. The remaining field QTLs were found in only the Canadian or the Mexican environment, not both. The QTL from Strongfield on 3A, , conferred seedling resistance to all races tested and had a significant effect in the field in Canada. This is the first report of and / as key components of genetic resistance in Canadian durum wheat. KASP markers were developed to detect for use in marker-assisted leaf rust resistance breeding. The susceptible parental lines contributed QTLs on 1A, 2B, and 5B that were effective in Mexican field tests and may be good targets to integrate into modern durum varieties to improve resistance to new durum virulent races.
硬粒小麦（）近年来受到由引起的新的致病性叶锈病的威胁。本研究旨在确定现代加拿大硬粒小麦品种 Strongfield 的叶锈病抗性。使用不同的 菌株，在幼苗期分别对 Strongfield 与六个四倍体小麦品系杂交产生的六个群体进行了测试。其中两个群体在加拿大和墨西哥进行了成株期叶锈病感染评估。通过 MapQTL 进行了逐步回归联合连锁数量性状位点（QTL）作图和分析。Strongfield 贡献了大多数检测到的 QTL，在田间试验中检测到了七个 QTL，并对幼苗抗性有八个 QTL 条件。来自 Strongfield 的 1B QTL ，对加拿大和墨西哥田间试验均有显著影响，与 / 对应。其余的田间 QTL 仅在加拿大或墨西哥环境中被发现，而不在两个环境中都被发现。Strongfield 位于 3A 上的 QTL ，赋予了对所有测试菌株的幼苗抗性，并在加拿大田间试验中具有显著效果。这是首次报道 和 / 是加拿大硬粒小麦遗传抗性的关键组成部分。开发了 KASP 标记来检测 ，用于标记辅助叶锈病抗性育种。感病亲本系在墨西哥田间试验中贡献了有效于 1A、2B 和 5B 的 QTL，可能是整合到现代硬粒小麦品种中以提高对新的硬粒小麦致病性菌株的抗性的良好目标。
