The classification of Sejroe group serovars of Leptospira interrogans with monoclonal antibodies.

Using the hybridoma technique we produced monoclonal antibodies to serovars of Leptospira interrogans. We focussed on serovar hardjo which is an important pathogen for humans and animals, and on other serovars of the Sejroe group. With combinations of monoclonals, characteristic patterns of agglutination were observed according to the specific pattern of antigenic determinants of that serovar. A set of 13 monoclonal antibodies was composed which allowed the classification of almost all serovars of the Sejroe group.