Institute of Physiological Chemistry, Faculty of Chemistry, University of Vienna, 1090 Vienna, Austria.
Vienna Doctoral School in Chemistry (DoSChem), University of Vienna, Währinger Str. 42, 1090 Vienna, Austria.
J Agric Food Chem. 2024 Jul 31;72(30):16749-16760. doi: 10.1021/acs.jafc.4c03264. Epub 2024 Jul 17.
Microalgae are promising sources of essential lipids, including omega-3 and omega-6 polyunsaturated fatty acids (n-3 and n-6 PUFA) and novel lipid metabolites like oxylipins. However, limited data exist on the oxylipin profile, its characterization, and the potential impact of the extraction process on these metabolites in microalgae. Thus, our study aimed to investigate the fatty acid and oxylipin profile of four microalgal species of interest (, , , and ) while also examining the impact of the extraction method, with a focus on developing a greener process using ultrasound-assisted extraction (UAE) and ethanol. The UAE method showed similar oxylipin profiles, generally yielding concentrations comparable to those of the conventional Folch method. In total, 68 oxylipins derived from n-3 and n-6 PUFA were detected, with the highest concentrations of n-3 oxylipins found in and and of n-6 oxylipins in . This study provides the most extensive oxylipin characterization of these microalgae species to date, offering insights into alternative extraction methods and opening new avenues for further investigation of the significance of oxylipins in microalgae.
微藻是有前途的必需脂质来源，包括ω-3 和 ω-6 多不饱和脂肪酸（n-3 和 n-6PUFA）和新型脂质代谢物，如氧化脂类。然而，关于微藻中氧化脂类的特征、其特征及其在提取过程中对这些代谢物的潜在影响的数据有限。因此，我们的研究旨在调查四种有兴趣的微藻物种（、、、和）的脂肪酸和氧化脂类谱，同时还研究了提取方法的影响，重点是使用超声辅助提取（UAE）和乙醇开发更环保的工艺。UAE 方法显示出相似的氧化脂类谱，通常产生的浓度与常规 Folch 方法相当。总共检测到 68 种源自 n-3 和 n-6PUFA 的氧化脂类，其中 n-3 氧化脂类在和中的浓度最高，n-6 氧化脂类在中最高。本研究迄今为止对这些微藻物种进行了最广泛的氧化脂类特征描述，为替代提取方法提供了深入了解，并为进一步研究氧化脂类在微藻中的意义开辟了新的途径。
