通过免疫荧光染色在幼稚 B 细胞、活化 B 细胞和记忆 B 细胞亚群中可视化表观遗传修饰和核体。

Visualizing Epigenetic Modifications and Nuclear Bodies by Immunofluorescence Staining in Naïve, Activated, and Memory B Cell Subsets.

机构信息

Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia.

Immunity Program, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

出版信息

Methods Mol Biol. 2024;2826:47-54. doi: 10.1007/978-1-0716-3950-4_4.

DOI:10.1007/978-1-0716-3950-4_4

PMID:39017884

Abstract

Immunofluorescence microscopy is a powerful technique using fluorescently labelled antibodies which can be used to visualize proteins in the nucleus. A key advantage of this method is that it can provide insight into the spatial organization and the localization of nuclear proteins, which can provide elucidation of their function. Here, we provide a protocol for immunofluorescence staining in the nucleus, which has successfully been used to visualize histone modifications and nuclear bodies in human and mouse B lymphocytes, using as few as 1 × 10-5 × 10 cells.

摘要

免疫荧光显微镜技术是一种使用荧光标记抗体的强大技术，可用于可视化细胞核中的蛋白质。该方法的一个主要优势是它可以提供有关核蛋白的空间组织和定位的深入了解，从而阐明其功能。在这里，我们提供了一种在细胞核中进行免疫荧光染色的方案，该方案已成功用于可视化人类和小鼠 B 淋巴细胞中的组蛋白修饰和核体，使用的细胞数少至 1×10-5×10 个。