Department of Food Science, Cornell University, Ithaca, New York, USA; email:
Annu Rev Anal Chem (Palo Alto Calif). 2024 Jul;17(1):393-410. doi: 10.1146/annurev-anchem-071323-084224.
Bacteriophages, which are viral predators of bacteria, have evolved to efficiently recognize, bind, infect, and lyse their host, resulting in the release of tens to hundreds of propagated viruses. These abilities have attracted biosensor developers who have developed new methods to detect bacteria. Recently, several comprehensive reviews have covered many of the advances made regarding the performance of phage-based biosensors. Therefore, in this review, we first describe the landscape of phage-based biosensors and then cover advances in other aspects of phage biology and engineering that can be used to make high-impact contributions to biosensor development. Many of these advances are in fields adjacent to analytical chemistry such as synthetic biology, machine learning, and genetic engineering and will allow those looking to develop phage-based biosensors to start taking alternative approaches, such as a bottom-up design and synthesis of custom phages with the singular task of detecting their host.
噬菌体是细菌的病毒捕食者，它们已经进化到能够高效地识别、结合、感染和裂解宿主，从而释放出数十到数百个增殖的病毒。这些能力吸引了生物传感器开发者，他们开发了新的方法来检测细菌。最近，有几篇综合评论涵盖了许多关于基于噬菌体的生物传感器性能的进展。因此，在这篇综述中，我们首先描述了基于噬菌体的生物传感器的现状，然后介绍了噬菌体生物学和工程学的其他方面的进展，这些进展可以为生物传感器的发展做出重大贡献。其中许多进展处于与分析化学相邻的领域，如合成生物学、机器学习和遗传工程，这将使那些希望开发基于噬菌体的生物传感器的人能够开始采用替代方法，例如使用具有检测其宿主这一单一任务的定制噬菌体进行自下而上的设计和合成。
