The University of Tennessee, 676 Dabney Hall, Knoxville, TN, 37996, USA,
Adv Biochem Eng Biotechnol. 2010;118:65-83. doi: 10.1007/10_2009_7.
Considered the most abundant organism on Earth, at a population approaching 10(31), bacteriophage, or phage for short, mediate interactions with myriad bacterial hosts that has for decades been exploited in phage typing schemes for signature identification of clinical, food-borne, and water-borne pathogens. With over 5,000 phage being morphologically characterized and grouped as to susceptible host, there exists an enormous cache of bacterial-specific sensors that has more recently been incorporated into novel bio-recognition assays with heightened sensitivity, specificity, and speed. These assays take many forms, ranging from straightforward visualization of labeled phage as they attach to their specific bacterial hosts to reporter phage that genetically deposit trackable signals within their bacterial hosts to the detection of progeny phage or other uniquely identifiable elements released from infected host cells. A comprehensive review of these and other phage-based detection assays, as directed towards the detection and monitoring of bacterial pathogens, will be provided in this chapter.
噬菌体被认为是地球上数量最多的生物，其种群数量接近 10(31)，与无数细菌宿主相互作用，几十年来一直被用于噬菌体分型方案，以鉴定临床、食源和水源病原体的特征。已经有超过 5000 种噬菌体被形态学特征化，并根据易感宿主进行分组，因此存在着大量细菌特异性传感器，这些传感器最近已被纳入新型生物识别检测中，提高了灵敏度、特异性和速度。这些检测方法有多种形式，从标记噬菌体附着到其特定细菌宿主的简单可视化，到在细菌宿主内遗传沉积可追踪信号的报告噬菌体，再到检测从感染宿主细胞释放的后代噬菌体或其他独特可识别的元素。本章将全面回顾这些以及其他基于噬菌体的检测方法，这些方法旨在检测和监测细菌病原体。
