College of Life Sciences, Jilin Agricultural University, Changchun 130118, Jilin, China.
College of Agronomy, Jilin Agricultural University, Changchun 130118, Jilin, China.
J Agric Food Chem. 2024 Jul 31;72(30):16889-16899. doi: 10.1021/acs.jafc.4c03084. Epub 2024 Jul 17.
Soybean ( [Linn.] Merr.) is an important oilseed crop. Although transcription factors (TFs) can coordinate the expression of mRNA and lncRNA, their coordination in the soybean oil synthesis pathway remains unclear. This study examined the interaction between the TF GmDof11 and and found that overexpression of led to an increase in the number of seeds, thousand seed weight, crude protein, hydrolysis amino acid, and soluble sugar. Additionally, it reduced the triglyceride and starch contents and affected the proportion of fatty acids, increasing the contents of palmitic acid, stearic acid, and linolenic acid. The yeast two-hybrid experiments revealed that GmDof11 interacts with GmBCCP1, GmLEC1b, and GmFAB2 proteins. In the RT-qPCR analysis of transgenic soybean roots, it was found that GmDof11 can activate the production of and work in conjunction with to oversee oil synthesis and nutrient storage. Our research provides robust theoretical evidence for a comprehensive resolution of TF- regulation in the soybean oil synthesis network.
大豆（[Linn.] Merr.）是一种重要的油料作物。尽管转录因子（TFs）可以协调 mRNA 和 lncRNA 的表达，但它们在大豆油脂合成途径中的协调作用尚不清楚。本研究检测了 TF GmDof11 和 的相互作用，发现过表达 导致种子数量增加，千粒重、粗蛋白、水解氨基酸和可溶性糖增加。此外，它降低了甘油三酯和淀粉的含量，并影响了脂肪酸的比例，增加了棕榈酸、硬脂酸和亚麻酸的含量。酵母双杂交实验表明，GmDof11 与 GmBCCP1、GmLEC1b 和 GmFAB2 蛋白相互作用。在转基因大豆根的 RT-qPCR 分析中发现，GmDof11 可以激活 的产生，并与 协同作用，监督油脂合成和养分储存。我们的研究为全面解析大豆油脂合成网络中的 TF 调控提供了有力的理论依据。
