PPGBiotec, Federal University of São Carlos, São Carlos, Brazil.
São Carlos Institute of Physics, University of São Paulo, São Paulo, Brazil.
J Biophotonics. 2024 Sep;17(9):e202400190. doi: 10.1002/jbio.202400190. Epub 2024 Jul 17.
Candida albicans biofilm can cause diseases that are resistant to conventional antifungal agents. Photodynamic (PDI), sonodynamic (SDI), and sonophotodynamic (SPDI) inactivation have arisen as promising antimicrobial strategies. This study evaluated these treatments mediated by curcumin against C. albicans biofilms. For this, C. albicans biofilms were submitted to PDI, SDI, or SPDI with different light and ultrasound doses, then, the viability assay was performed to measure the effectiveness. Finally, a mathematical model was suggested to fit acquired experimental data and understand the synergistic effect of light and ultrasound in different conditions. The results showed that SPDI, PDI, and SDI reduced the viability in 6 ± 1; 1 ± 1; and 2 ± 1 log, respectively, using light at 60 J/cm, ultrasound at 3 W/cm, and 80 μM of curcumin. The viability reduction was proportional to the ultrasound and light doses delivered. These results encourage the use of SPDI for the control of microbial biofilm.
白色念珠菌生物膜可导致对抗生素有耐药性的疾病。光动力（PDI）、声动力（SDI）和声敏动力（SPDI）灭活已成为有前途的抗菌策略。本研究评估了姜黄素介导的这些治疗方法对白色念珠菌生物膜的作用。为此，将白色念珠菌生物膜进行 PDI、SDI 或 SPDI 处理，使用不同的光和超声剂量，然后进行存活率测定以测量有效性。最后，提出了一个数学模型来拟合获得的实验数据，并理解在不同条件下光和超声的协同作用。结果表明，使用 60 J/cm 的光、3 W/cm 的超声和 80 μM 的姜黄素，SPDI、PDI 和 SDI 分别将生物膜的存活率降低了 6±1、1±1 和 2±1 log。存活率的降低与所施加的超声和光剂量成正比。这些结果鼓励使用 SPDI 来控制微生物生物膜。
