Ashi Tarek, Kharouf Naji, Etienne Olivier, Cournault Bérangère, Klienkoff Pierre, Gribova Varvara, Haikel Youssef
Department of Biomaterials and Bioengineering, University of Strasbourg, INSERM UMR_S 1121, Strasbourg, France.
Department of Endodontics and Conservative Dentistry, Faculty of Dental Medicine, University of Strasbourg, Strasbourg, France.
Eur J Dent. 2025 Feb;19(1):240-247. doi: 10.1055/s-0044-1788319. Epub 2024 Jul 19.
The purpose of the present study was to investigate the created roughness and cell attachment of intact teeth (C), obturated teeth with bioceramic (BR), or epoxy resin (AH) after root resection using piezoelectric ultrasonic and carbide bur.
Three groups of first mandibular premolars were used in the present study: control group (without any preparation or obturation) (C); second group was obturated with an epoxy resin sealer (AH, AH Plus Jet); and finally, the third one was obturated with a bioceramic sealer (BR, BioRoot RCS). All teeth were incubated for 4 months at 37°C. After that, the samples were sectioned using tungsten carbide bur or piezoelectric ultrasonic. Roughness and then cell attachment of periodontal ligament cells on the sectioned surfaces were investigated by profilometer and confocal microscope, respectively.
Data were statistically analyzed using one-way analysis of variance.
After root resection, no significant difference was found between the roughness among the three groups sectioned using the piezoelectric technique ( > 0.05). In contrast, concerning the sectioned samples by burs, C demonstrated a rougher surface compared with BR ( < 0.05). There was a significant higher cell attachment in BR compared with AH in the piezoelectric groups ( = 0.047), while no statistically significant difference was found between the groups sectioned with bur ( > 0.05).
Dentists are now focused on the use of calcium silicate-based sealers due to their bioactivity. The present study advises dentists to use bioceramic sealer which could improve the dentin characteristics which ameliorate the cell attachment.
本研究旨在调查在使用压电超声和硬质合金车针进行牙根切除后，完整牙齿（C组）、用生物陶瓷封闭的牙齿（BR组）或用环氧树脂封闭的牙齿（AH组）的表面粗糙度及细胞附着情况。
本研究使用了三组下颌第一前磨牙：对照组（未作任何预备或封闭）（C组）；第二组用环氧树脂封闭剂（AH、AH Plus Jet）进行封闭；最后，第三组用生物陶瓷封闭剂（BR、BioRoot RCS）进行封闭。所有牙齿在37°C下孵育4个月。之后，使用硬质合金车针或压电超声对样本进行切片。分别通过轮廓仪和共聚焦显微镜研究切片表面的粗糙度以及牙周膜细胞的附着情况。
数据采用单因素方差分析进行统计学分析。
牙根切除后，使用压电技术切片的三组之间粗糙度无显著差异（P>0.05）。相比之下，对于用车针切片的样本，C组的表面比BR组更粗糙（P<0.05）。在压电组中，BR组的细胞附着显著高于AH组（P=0.047），而用车针切片的各组之间未发现统计学显著差异（P>0.05）。
由于其生物活性，牙医目前专注于使用硅酸钙基封闭剂。本研究建议牙医使用生物陶瓷封闭剂，其可改善牙本质特性，进而改善细胞附着。
