Bounous G, Shenouda N, Kongshavn P A, Osmond D G
J Nutr. 1985 Nov;115(11):1409-17. doi: 10.1093/jn/115.11.1409.
The effect of 20 g/100 g dietary lactalbumin (L) or casein (C) diets or a nonpurified (NP) diet on the immune responsiveness of C57Bl/6J, C3H/HeJ and BALB/cJ mice has been investigated by measuring the response to the T cell-independent antigen, TNP-Ficoll. To investigate the possible influence of dietary protein type on the supply of B lymphocytes, bone marrow lymphocyte production has been examined by a radioautographic assay of small lymphocyte renewal and an immunofluorescent stathmokinetic assay of pre-B cells and their proliferation. The humoral response of all mice fed the L diet was found to be higher than that of mice fed the C diet or nonpurified diet. A similar pattern of dietary protein effect in (CBA/N X DBA/2J) F1 mice carrying the xid defect was observed following challenge with sheep red blood cells (SRBC). An even greater enhancing effect of dietary L was noted in normal (DBA/2J X CBA/N) F1 mice after immunization with SRBC, but in contrast, the normal large-scale production of B lymphocytes in mouse bone marrow was independent of the type of dietary protein. Dietary protein type did not affect blood level of minerals and trace metals. The free plasma amino acid profile essentially conformed to the amino acid composition of the ingested protein, suggesting that the changes in plasma amino acid profile might be a crucial factor in diet-dependent enhancement or depression of the B-cell response.(ABSTRACT TRUNCATED AT 250 WORDS)
通过测量对非T细胞依赖性抗原TNP-菲可（TNP-Ficoll）的反应，研究了含20 g/100 g乳白蛋白（L）或酪蛋白（C）的日粮或非纯化（NP）日粮对C57Bl/6J、C3H/HeJ和BALB/cJ小鼠免疫反应性的影响。为了研究日粮蛋白质类型对B淋巴细胞供应的可能影响，通过小淋巴细胞更新的放射自显影测定法以及前B细胞及其增殖的免疫荧光静止动力学测定法，检测了骨髓淋巴细胞生成情况。发现所有饲喂L日粮的小鼠的体液反应高于饲喂C日粮或非纯化日粮的小鼠。在用绵羊红细胞（SRBC）攻击后，观察到携带xid缺陷的（CBA/N×DBA/2J）F1小鼠中日粮蛋白质效应的类似模式。在用SRBC免疫后，正常的（DBA/2J×CBA/N）F1小鼠中日粮L的增强作用甚至更大，但相反，小鼠骨髓中B淋巴细胞的正常大规模生成与日粮蛋白质类型无关。日粮蛋白质类型不影响矿物质和微量元素的血液水平。游离血浆氨基酸谱基本符合摄入蛋白质的氨基酸组成，这表明血浆氨基酸谱的变化可能是日粮依赖性增强或抑制B细胞反应的关键因素。（摘要截短于250字）
