Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, 364 002, Gujarat, India.
Plant Omics Division, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Gijubhai Badheka Marg, Bhavnagar, 364 002, Gujarat, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Plant Physiol Biochem. 2024 Sep;214:108928. doi: 10.1016/j.plaphy.2024.108928. Epub 2024 Jul 10.
The RING-type E3 ligases play a significant role in stress signaling, primarily through post-translational regulation. Ubiquitination is a crucial post-translational modification that regulates the turnover and activity of proteins. The overexpression of AlRabring7, RING-HC E3 Ub ligase in tobacco provides insights into the regulation of salinity and ABA signaling in transgenic tobacco. The seed germination potential of AlRabring7 transgenics was higher than WT, with NaCl and ABA treatments. The transgenics showed improved morpho-physio-biochemical parameters in response to salinity and ABA treatments. The photosynthetic pigments, soluble sugars, reducing sugars and proline increased in transgenics in response to NaCl and ABA treatments. The decreased ROS accumulation in transgenics on NaCl and ABA treatments can be co-related to improved activity of enzymatic and non-enzymatic antioxidants. The potential of transgenics to maintain ABA levels with ABA treatment, highlights the active participation of ABA feedback loop mechanism. Interestingly, the ability of AlRabring7 transgenics to upregulate Rab7 protein, suggests its role in facilitating vacuolar transport. Furthermore, the improved potassium accumulation and reduced sodium content indicate an efficient ion regulation mechanism in transgenic plants facilitating higher stomatal opening. The expression of downstream ion transporter (NbNHX1 and NbVHA1), ABA signaling (NbABI2 and NbABI5) and vesicle trafficking (NbMON1) responsive genes were upregulated with stress. The present study, reports that AlRabring7 participates in maintaining vacuolar transport, ion balance, ROS homeostasis, stomatal regulation through activation of Rab7 protein and regulation of downstream stress-responsive during stress. This emphasizes the potential of AlRabring7 gene for improved performance and resilience in challenging environments.
RING 型 E3 连接酶在应激信号转导中起着重要作用，主要通过翻译后调控。泛素化是一种重要的翻译后修饰，调节蛋白质的周转和活性。烟草中 AlRabring7 的过表达，即 RING-HC E3 Ub 连接酶，为研究盐度和 ABA 信号转导在转基因烟草中的调控提供了线索。AlRabring7 转基因的种子发芽潜能高于 WT，在 NaCl 和 ABA 处理下更是如此。与 WT 相比，转基因在应对盐度和 ABA 处理时表现出更好的形态、生理、生物化学参数。在 NaCl 和 ABA 处理下，转基因中的光合色素、可溶性糖、还原糖和脯氨酸增加。在 NaCl 和 ABA 处理下，转基因中 ROS 积累减少与抗氧化酶活性的提高有关。与 ABA 处理相比，转基因维持 ABA 水平的潜力突出了 ABA 反馈环机制的积极参与。有趣的是，AlRabring7 转基因上调 Rab7 蛋白的能力表明其在促进液泡运输中的作用。此外，钾积累增加和钠含量降低表明转基因植物中存在有效的离子调节机制，促进了更高的气孔开度。下游离子转运体（NbNHX1 和 NbVHA1）、ABA 信号转导（NbABI2 和 NbABI5）和囊泡运输（NbMON1）响应基因的表达在胁迫下上调。本研究报告称，AlRabring7 通过激活 Rab7 蛋白和调节下游应激响应基因参与维持液泡运输、离子平衡、ROS 稳态、气孔调节，这强调了 AlRabring7 基因在挑战性环境下提高性能和恢复力的潜力。
