Feldner J, Bredt W, Kahane I
Infect Immun. 1979 Jul;25(1):60-7. doi: 10.1128/iai.25.1.60-67.1979.
The human pathogen Mycoplasma pneumoniae adheres to a variety of cells, including erythrocytes. A hemadsorption technique was developed to quantitate adherence by photometric measurement of lysates of erythrocytes that attached to sheets of M. pneumoniae grown in cups of Linbro plates. Attachment of sheep erythrocytes (SE) increased with higher ionic strength, was unaffected by minor pH variations (6 to 9), and was blocked by anti-M. pneumoniae antiserum, but was not inhibited by a variety of sugars, amino acids, and bovine serum albumin. The reaction was time and temperature dependent. The temperature curve showed peaks at 14 and 28 degrees C with untreated SE but only one peak at about 38 degrees C with glutaraldehyde-treated SE. The temperature dependence indicated involvement of either metabolic or membrane activities in the binding process. Trypsin treatment of the M. pneumoniae sheet abolished adherence of SE but was only partially effective with human erythrocytes and noneffective with rabbit erythrocytes. The binding capacity of the mycoplasma cells for SE was restored by incubation in growth medium for 3 to 4 h; this restoration was inhibited by 10 mug of chloramphenicol per ml. Neuraminidase treatment of SE removed their attachment capacity but had no effect on attachment of rabbit erythrocytes and only a slight effect on attachment of human erythrocytes. Pretreatment of M. pneumoniae with neuraminic acid partially blocked the adherence of SE, whereas rabbit erythrocyte attachment was not affected. Attached SE could be detached by trypsin, but not by neuraminidase. For human and rabbit erythrocytes, the results suggest binding mechanisms other than the interaction between neuraminidase-sensitive receptors and protein-containing binding sites shown for SE.
人类病原体肺炎支原体可黏附于多种细胞，包括红细胞。人们开发了一种血细胞吸附技术，通过光度测量附着在林伯板培养杯中的肺炎支原体菌膜上的红细胞裂解物，来定量黏附情况。绵羊红细胞（SE）的黏附随着离子强度的升高而增加，不受微小pH变化（6至9）的影响，并被抗肺炎支原体抗血清阻断，但不受多种糖类、氨基酸和牛血清白蛋白的抑制。该反应具有时间和温度依赖性。温度曲线显示，未处理的SE在14和28摄氏度时有峰值，而经戊二醛处理的SE在约38摄氏度时只有一个峰值。温度依赖性表明结合过程涉及代谢或膜活性。用胰蛋白酶处理肺炎支原体菌膜可消除SE的黏附，但对人红细胞仅部分有效，对兔红细胞则无效。支原体细胞对SE的结合能力在生长培养基中孵育3至4小时后得以恢复；每毫升10微克氯霉素可抑制这种恢复。用神经氨酸酶处理SE可消除其黏附能力，但对兔红细胞的黏附无影响，对人红细胞的黏附只有轻微影响。用神经氨酸预处理肺炎支原体可部分阻断SE的黏附，而兔红细胞的黏附不受影响。附着的SE可用胰蛋白酶分离，但不能用神经氨酸酶分离。对于人和兔红细胞，结果表明其结合机制不同于SE所显示的神经氨酸酶敏感受体与含蛋白质结合位点之间的相互作用。
