Feldner J, Bredt W
J Gen Microbiol. 1983 Mar;129(3):841-8. doi: 10.1099/00221287-129-3-841.
Haemadsorption-negative mutants of Mycoplasma pneumoniae were isolated which varied in their capacity to adsorb erythrocytes of various animal species suggesting adherence to erythrocytes is mediated by different binding mechanisms. Trypsin treatment of the wild-type strain resulted in loss of haemadsorbing activity; several polypeptides, some of which regenerated with haemadsorbing activity following further incubation, were also trypsin sensitive. The haemadsorption-negative mutants could be divided into two groups according to their polypeptide pattern. In the first group (11 mutants) the PAGE pattern was identical to that of the wild-type strain. The second group comprised 7 mutants which differed from the wild-type by lack of one or more polypeptides with molecular weights of 190 000, 90 000 or 40 000. During growth attachment to glass was weak or absent in the mutants. Surface hydrophobicity as measured by hydrophobic-interaction chromatography was nearly comparable in mutants and parent strain.
分离出肺炎支原体的血细胞吸附阴性突变体，它们吸附各种动物物种红细胞的能力各不相同，这表明对红细胞的黏附是由不同的结合机制介导的。用胰蛋白酶处理野生型菌株会导致血细胞吸附活性丧失；几种多肽，其中一些在进一步孵育后恢复了血细胞吸附活性，它们对胰蛋白酶也敏感。根据多肽图谱，血细胞吸附阴性突变体可分为两组。第一组（11个突变体）的聚丙烯酰胺凝胶电泳图谱与野生型菌株相同。第二组包括7个突变体，它们与野生型的不同之处在于缺少一种或多种分子量为190000、90000或40000的多肽。在生长过程中，突变体对玻璃的附着较弱或没有附着。通过疏水相互作用色谱法测定的表面疏水性在突变体和亲本菌株中几乎相当。
