Human Genetics Laboratory, Faculty of Medicine of Tunis, Tunis El Manar University, Tunis, Tunisia.
Faculty of Medicine of Tunis, Aziza Othmena Hospital, Tunis El Manar University, Tunis, Tunisia.
Med Oncol. 2024 Jul 22;41(8):206. doi: 10.1007/s12032-024-02453-y.
Cure rates for acute myeloid leukemia (AML) remain suboptimal; thus, new treatment strategies are needed for this deadly disease. Artemisia campestris leaves hold significant value in traditional medicine. Despite extensive research conducted on this plant globally, the specific anti-AML properties of the leaves have received limited investigation. This study aims to explore the potential anti-leukemic activities of the ethyl acetate extract derived from Artemisia campestris (EAEAC), using mononuclear cells from bone marrow of thirteen AML patients. To this end, cytotoxic effects were evaluated using the MTT assay, and the mechanisms of cell death were investigated through various methods, including propidium iodide staining, annexin V/propidium iodide double staining, mitochondrial depolarization, and caspase-3/7 activation assays. Results demonstrated that EAEAC induced cell apoptosis by increasing DNA fragmentation, causing mitochondrial depolarization, and activating caspases 3/7. On the other hand, we assessed EAEAC's effect on two leukemia stem cell subpopulations, with results suggesting a potential decrease in their frequencies (three/five patients).
急性髓系白血病 (AML) 的治愈率仍然不理想；因此，这种致命疾病需要新的治疗策略。青蒿叶在传统医学中具有重要价值。尽管全球范围内对该植物进行了广泛的研究，但对其叶子的具体抗 AML 特性的研究却很有限。本研究旨在探讨从青蒿（EAEAC）中提取的乙酸乙酯提取物的潜在抗白血病活性，使用来自 13 名 AML 患者骨髓的单核细胞。为此，通过 MTT 测定评估细胞毒性作用，并通过各种方法（包括碘化丙啶染色、膜联蛋白 V/碘化丙啶双重染色、线粒体去极化和 caspase-3/7 激活测定）研究细胞死亡的机制。结果表明，EAEAC 通过增加 DNA 片段化、导致线粒体去极化和激活 caspase 3/7 诱导细胞凋亡。另一方面，我们评估了 EAEAC 对两种白血病干细胞亚群的影响，结果表明其频率可能降低（三/五名患者）。
