Chou J, Lemaux P G, Casadaban M J, Cohen S N
Nature. 1979;282(5741):801-6. doi: 10.1038/282801a0.
Fusions that bring lac gene expression under the control of transcriptional and translational signals within the Tn3 element have been used to identify and characterise a Tn3-encoded 'transposase' (Tnp) peptide of MW 100,000 essential for transposition. The gene specifying this product is regulated by the Tn3 repressor protein and is part of a bidirectional genetic unit that includes the repressor gene.
已利用使lac基因表达受Tn3元件内转录和翻译信号控制的融合技术来鉴定和表征一种分子量为100,000的Tn3编码“转座酶”(Tnp)肽,该肽对转座至关重要。指定此产物的基因受Tn3阻遏蛋白调控,并且是一个双向遗传单位的一部分,该单位包括阻遏基因。