Piette J, Cunin R, Boyen A, Charlier D, Crabeel M, Van Vliet F, Glansdorff N, Squires C, Squires C L
Nucleic Acids Res. 1982 Dec 20;10(24):8031-48. doi: 10.1093/nar/10.24.8031.
The nucleotide sequence of the control region of the divergent argECBH operon has been established in the wild type and in mutants affecting expression of these genes. The argE and argCBH promoters face each other and overlap with an operator region containing two domains which may act as distinct repressor binding sites. A long leader sequence - not involved in attenuation - precedes argCBH. Overlapping of the argCBH promoter and the region involved in ribosome mobilization for argE translation explains the dual effect of some mutations. Mutations causing semi-constitutive expression of argE improve putative promoter sequences within argC. Implications of these results regarding control mechanisms in amino acid biosynthesis and their evolution are discussed.
已确定了野生型以及影响这些基因表达的突变体中， divergent argECBH操纵子控制区的核苷酸序列。argE和argCBH启动子相对，且与一个含有两个结构域的操纵区重叠，这两个结构域可能作为不同的阻遏物结合位点。在argCBH之前有一个长的前导序列——不参与衰减作用。argCBH启动子与参与argE翻译的核糖体动员区域的重叠解释了一些突变的双重效应。导致argE半组成型表达的突变改善了argC内的假定启动子序列。讨论了这些结果对于氨基酸生物合成控制机制及其进化的意义。
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