Song Hou-Pan, Ou Chen, Xiong Meng, Jiang Peng-Fei, Zeng Mei-Yan, Lu Jing, Peng Jun, Zhou Ya-Sha, Yang Yi-Jing, Peng Qing-Hua
Hunan Provincial Key Laboratory of Traditional Chinese Medicine Diagnostics, Hunan University of Chinese Medicine Changsha 410208, China Institute of Traditional Chinese Medicine Diagnostics, Hunan University of Chinese Medicine Changsha 410208, China College of Traditional Chinese Medicine, Hunan University of Chinese Medicine Changsha 410208, China.
Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye, Ear, Nose and Throat Diseases, Hunan University of Chinese Medicine Changsha 410208, China.
Zhongguo Zhong Yao Za Zhi. 2024 Jun;49(11):3040-3049. doi: 10.19540/j.cnki.cjcmm.20240115.707.
This study aims to explore the effect of Lycii Fructus and Salviae Miltiorrhizae Radix et Rhizoma(LFSMR), a drug pair possesses the function of nourishing Yin, promoting blood circulation, and brightening the eyes, in treating retinitis pigmentosa(RP)by inhibiting the gliosis of Müller cells(MCs) and inducing their reprogramming and differentiation into various types of retinal nerve cells. Twelve C57 mice were used as the normal control group, and 48 transgenic RP(rd10) mice were randomly divided into the model group, positive control group, and low and high dose LFSMR groups, with 12 mice in each group. HE staining was used to detect pathological changes in the retina, and an electroretinogram was used to detect retinal function. Retinal optical coherence tomography was used to detect retinal thickness and perform fundus photography, and laser speckle perfusion imaging was used to detect local retinal blood flow. Digital PCR was used to detect gene expression related to retinal nerve cells, and immunofluorescence was used to detect protein expression related to retinal nerve cells. LFSMR could significantly improve the pathological changes, increase the amplitude of a and b waves, increase the retinal thickness, restore retinal damage, and increase retinal blood flow in mice with RP lesions. LFSMR could also significantly inhibit the m RNA expression of the glial fibrillary acidic protein( GFAP) during the pathogenesis of RP and upregulate m RNA expression of sex determining region Y box protein 2(SOX2), paired box protein 6(Pax6),rhodopsin, protein kinase C-α(PKCα), syntaxin, and thymic cell antigen 1. 1(Thy1. 1). LFSMR could significantly inhibit GFAP protein expression and enhance protein expression of SOX2, Pax6, rhodopsin, PKCα, syntaxin, and Thy1. 1. It could also reverse the pathological changes in the retina of rd10 mice, improve retinal function and fundus performance, increase retinal thickness, enhance local retinal blood flow, and exert therapeutic effects on RP. The mechanism of action of LFSMR may be related to inhibiting the gliosis of MCs and promoting their reprogramming and differentiation into various types of retinal nerve cells.
本研究旨在探讨枸杞与丹参这一具有滋阴、活血化瘀、明目功效的药对，通过抑制Müller细胞（MCs）的胶质化并诱导其重编程和分化为各种类型的视网膜神经细胞，对视网膜色素变性（RP）的治疗作用。选取12只C57小鼠作为正常对照组，将48只转基因RP（rd10）小鼠随机分为模型组、阳性对照组、低剂量和高剂量枸杞丹参药对组，每组12只。采用苏木精-伊红（HE）染色检测视网膜病理变化，用电视网膜图检测视网膜功能。用视网膜光学相干断层扫描检测视网膜厚度并进行眼底照相，用激光散斑血流成像检测局部视网膜血流。采用数字PCR检测与视网膜神经细胞相关的基因表达，用免疫荧光检测与视网膜神经细胞相关的蛋白表达。枸杞丹参药对可显著改善RP病变小鼠的病理变化，增加a波和b波振幅，增加视网膜厚度，修复视网膜损伤，增加视网膜血流。枸杞丹参药对还可在RP发病过程中显著抑制胶质纤维酸性蛋白（GFAP）的mRNA表达，并上调性别决定区Y框蛋白2（SOX2）、配对盒蛋白6（Pax6）、视紫红质、蛋白激酶C-α（PKCα）、 syntaxin和胸腺细胞抗原1.1（Thy1.1）的mRNA表达。枸杞丹参药对可显著抑制GFAP蛋白表达，增强SOX2、Pax6、视紫红质、PKCα、syntaxin和Thy1.1的蛋白表达。它还可逆转rd10小鼠视网膜的病理变化，改善视网膜功能和眼底表现，增加视网膜厚度，增强局部视网膜血流，对RP发挥治疗作用。枸杞丹参药对的作用机制可能与抑制MCs的胶质化并促进其重编程和分化为各种类型的视网膜神经细胞有关。
