State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Stress Biology, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, Guangdong, PR China.
Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006471.
Two strains, designated as SYSU M80004 and SYSU M80005, were isolated from water sampled in the Pearl River Estuary, Guangzhou, Guangdong, PR China. The strains were Gram-stain-negative and aerobic. Strain SYSU M80004 could grow at pH 6.0-8.0 (optimum, pH 7.0), 22-30 °C (optimum, 28 °C) and in the presence of 0-1 % NaCl (w/v; optimum 0 %). Strain SYSU M80005 could grow at pH 6.0-8.0 (optimum, pH 7.0), 4-37 °C (optimum, 28 °C) and in the presence of 0-1 % NaCl (w/v; optimum 0%). Both strains contained MK-6 as the predominant menaquinone. C and iso-C were identified as the major fatty acids (>10 %) of strain SYSU M80004 while strain SYSU M80005 contained iso-C and iso-C 3-OH as major fatty acids. Phosphatidylethanolamine was present as the major polar lipid in both strains. The average nucleotide identity and digital DNA-DNA hybridization values between these two strains and their closest relatives were 73.5-79.3 % and 19.6-23.2 %, respectively. Phylogenetic analysis based on the 16S rRNA gene and genomic sequences indicated they belonged to the genus . Therefore, on the basis of phenotypic, physiological, chemotaxonomic and genomic evidence, two novel species, sp. nov. (type strain=SYSU M80004=CDMCC 1.4522=KCTC 102268) and sp. nov. (type strain=SYSU M80005=CGMCC 1.4523= KCTC 102269) are proposed.
两株菌株，分别命名为 SYSU M80004 和 SYSU M80005，从中国广东省广州珠江口采集的水样中分离得到。这些菌株革兰氏阴性，需氧。SYSU M80004 株可在 pH 值 6.0-8.0（最佳 pH 值为 7.0）、22-30°C（最佳温度为 28°C）和 0-1%NaCl（w/v；最佳为 0%）的条件下生长。SYSU M80005 株可在 pH 值 6.0-8.0（最佳 pH 值为 7.0）、4-37°C（最佳温度为 28°C）和 0-1%NaCl（w/v；最佳为 0%）的条件下生长。两株菌均以 MK-6 为主要类异戊二烯醌。SYSU M80004 株的主要脂肪酸（>10%）为 C 和 iso-C，而 SYSU M80005 株的主要脂肪酸为 iso-C 和 iso-C 3-OH。磷脂酰乙醇胺是两株菌的主要极性脂。这两株菌与其最接近的亲缘菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 73.5-79.3%和 19.6-23.2%。基于 16S rRNA 基因和基因组序列的系统发育分析表明，它们属于 属。因此，基于表型、生理、化学分类和基因组证据，提出了两个新种，即 sp. nov.（模式菌株=SYSU M80004=CDMCC 1.4522=KCTC 102268）和 sp. nov.（模式菌株=SYSU M80005=CGMCC 1.4523=KCTC 102269）。
