新种，一种从南海分离得到的新型细菌。

sp. nov., a novel bacterium isolated from the South China Sea.

机构信息

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.

College of Life Sciences, Henan Normal University, Xinxiang, 453007, PR China.

出版信息

Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006388.

DOI:10.1099/ijsem.0.006388

PMID:38747701

Abstract

Two Gram-stain-negative strains, designed SYSU M86414 and SYSU M84420, were isolated from marine sediment samples of the South China Sea (Sansha City, Hainan Province, PR China). These strains were aerobic and could grow at pH 6.0-8.0 (optimum, pH 7.0), 4-37 °C (optimum, 28 °C), and in the presence of 0-10 % NaCl (w/v; optimum 3 %). The predominant respiratory menaquinone of strains SYSU M86414 and SYSU M84420 was MK-6. The primary cellular polar lipid was phosphatidylethanolamine. The major cellular fatty acids (>10 %) in both strains were iso-C, iso-C G, and iso-C 3-OH. The DNA G+C content of strains SYSU M86414 and SYSU M84420 were both 42.10 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and core genes indicated that these novel strains belonged to the genus and strain SYSU M86414 showed the highest 16S rRNA gene sequence similarity to JCM 11811 (98.83 %), followed by BC31-1-A7 (98.62 %), while strain SYSU M84420 had highest 16S rRNA gene sequence similarity to JCM 11811 (98.76 %) and BC31-1-A7 (98.55 %). Based on the results of polyphasic analyses, strains SYSU M86414 and SYSU M84420 should be considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the proposed novel isolate is SYSU M86414 (=GDMCC 1.3806=KCTC 102040).

摘要

两株革兰氏阴性菌株，设计为 SYSU M86414 和 SYSU M84420，从中国南海（海南省三沙市）的海洋沉积物样本中分离得到。这些菌株是需氧的，可以在 pH 值 6.0-8.0（最佳，pH 值 7.0）、4-37°C（最佳，28°C）和 0-10%NaCl（w/v；最佳 3%）的存在下生长。菌株 SYSU M86414 和 SYSU M84420 的主要呼吸menaquinone 是 MK-6。主要的细胞质极性脂质是磷脂酰乙醇胺。在这两个菌株中，主要的细胞脂肪酸（>10%）是 iso-C、iso-C G 和 iso-C 3-OH。菌株 SYSU M86414 和 SYSU M84420 的 DNA G+C 含量均为 42.10 mol%。基于 16S rRNA 基因序列和核心基因的系统发育分析表明，这些新菌株属于属，菌株 SYSU M86414 与 JCM 11811（98.83%）的 16S rRNA 基因序列相似度最高，其次是 BC31-1-A7（98.62%），而菌株 SYSU M84420 与 JCM 11811（98.76%）和 BC31-1-A7（98.55%）的 16S rRNA 基因序列相似度最高。基于多相分析的结果，菌株 SYSU M86414 和 SYSU M84420 应被视为属的一个新种，建议将其命名为 sp. nov.。提议的新分离株的模式菌株是 SYSU M86414（=GDMCC 1.3806=KCTC 102040）。